Dataset ID: 11
Dataset Details
Study Design
| Study Design Background |
To synthesis and evaluation of a PSMA-617-targeted dual NIRF PDTprobe conceived using IRDye700DX as photosensitizer and FGS NIR dye.
|
|---|---|
| Study Description |
Probe conceived using IRDye700DX as photosensitizer and FGS NIR dye
|
| Study Type |
Preclinical
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| Study Subtype |
In vivo ex vivo
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Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
A Novel PSMA-Targeted Probe for NIRF-Guided Surgery and Photodynamic Therapy: Synthesis and Preclinical Validation
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| Paper Authors |
Capozza, Martina; Stefania, Rachele; Dinatale, Valentina; Bitonto, Valeria; Conti, Laura; Grange, Cristina; Skovronova, Renata; Terreno, Enzo
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| Affiliation |
Molecular & Preclinical Imaging Centers, Department of Molecular Biotechnology and Health Sciences, University of Torino, 10126 Torino, Italy ; Molecular Biotechnology Center, Department of Medical Science, University of Torino, 10126 Torino, Italy; Molecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, 10126 Torino, Italy
|
| Paper Journal |
IJMS
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| Paper Year |
2022
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| Paper DOI | |
| Open Access |
Yes
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Study Component
| Multi Modality Images |
Yes
|
|---|---|
| Imaging Modality |
MRI, OI
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| Imaging Sub Modality |
T2w and fluorescent imaging (filter pair: 640 720 nm)
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| Radiation |
No
|
| Imaging Coverage |
Body
|
| Imaging Target |
PSMA
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Dataset Information
| Institution |
UniTO
|
|---|---|
| PI |
Enzo Terreno
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| Country Of Institution |
Italy
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| Imaging Facility |
Laboratory of molecular imaging for small animal
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| Euro Bio Imaging Node |
MMMI
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| Country Of Imaging Facility |
Italy
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| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
5
|
|---|---|
| Types Of Groups |
1nmol PSMA+ inj biod; 5nmol PSMA+ inj biod; 5 nmol PSMA- biod; PSMA+ PDT treated; PSMA+ Ctrl
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| Overall Sample Size |
25
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| Disease Model |
Cancer
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| Organ Or Tissue |
Prostate
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| Sample Size For Each Group |
5, 5, 5, 5, 5
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| Randomization |
Mice were randomly divided into treted and control group
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| Blinding |
No
|
| Outcome Measures |
Fluorescent uptake, tumor volume
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| Statistical Methods |
Anova and t-test
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| Species |
Mice
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| Strain |
Athymic nude
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| Immune Status |
Immunodeficient
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| Sex |
Male
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| Age |
5 weeks
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| Age At Start Experiment |
6 weeks
|
| Age At Scanning Experiment S |
Older than 8 weeks
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| Weight |
20g
|
| Weight At Start Experiment |
20
|
| Weight At End Experiment |
20
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| Source Of Animals |
Envigo
|
| Registry Number Of Animal Authorization |
229 2016
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Experimental Procedures
| Pharmacological Drug |
IRDye700DX-PSMA
|
|---|---|
| Company |
Unito
|
| Formulation |
PBS
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| Drug Dose |
1-5 nmol mice
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| Site Or Route Of Administration |
Iv
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| Frequency Of Administration |
Once
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| Vehicle Or Carrier Solution Formulation |
PBS
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| Blood Sampling |
No
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| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia Type |
Intramuscolar
|
| Duration |
45
|
| Anesthesia Drugs |
Zoletil Rompun
|
| Anesthesia Dose |
20 mg kg tiletamine zolazepam (Zoletil 100; Virbac, Milan, Italy) and 5 mg kg xylazine (Rompun; Bayer, Milan, Italy).
|
| Euthanasia |
Yes
|
| Method |
Cervival dislocation
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| Histology |
Yes
|
| Tissues Collected Post Euthanasia |
Tumor
|
| Timing Of Collection |
Different time points
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| Histological Procedure |
Optical cutting temperature (OCT) compound
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| Name Of Reagent S |
Anti-CD68, anti PSMA, anti CD31,
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| Frequency Of Imaging |
2 times week MRI
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| Timing Of Imaging |
Three weeks after inoculation
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| Overall Scan Length |
20 min
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
No
|
| Cell Line |
PC3-PIP and PC3-FLU
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| Provenance |
THE JOHNS HOPKINS UNIVERSITY
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| Modified Cell Line |
Yes
|
| Type Of Genetic Modification |
Plasmid
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| Gene Modified |
PSMA
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| Cell Injection Route |
Subcutaneous
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| Number Of Cells |
2x10^6
|
| Housing Room |
SPF and not SPF
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| Dietary Intervention |
Diet for OI
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Image Acquisition
| Instrument Vendor |
Bruker for MRI and Perkin Elmer for OI
|
|---|---|
| Instrument Type |
7 T Bruker Pharmascan, IVIS spectrum
|
| Instrument Specifics |
7T
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| Raw Data |
Yes
|
| QA/QC |
No
|
Image Data
| Image Scale |
Average radiance efficiency for OI
|
|---|---|
| Format Compression |
Raw
|
| Dimensions |
2D
|
| Overall Number Of Images |
32 x MRI; 7 x OI
|
| Field Of View |
30x30 for MRI; D for Ivis
|
| Dimension Extents |
128x128
|
| Size Description |
30x30x2 mm
|
| Image Processing Methods |
Default
|
Analyzed Data
| Analysis Result Type |
Numerical
|
|---|---|
| Data Used For Analysis |
ROI
|
| Analysis Method And Details |
Manual
|
| File Format Of Result File Csv Json Txt Xlsx |
Xlsx
|
| Status |
Complete
|
| Updated Year |
2023
|