Dataset ID: 13
Dataset Details
Study Design
| Study Design Background |
This study investigated the potential of 1H relaxometry and 23 Na MRI to monitor water fluxes and dysregulation of Na+ homeostasis as novel target for therapeutic intervention. The effect of a forced Na+ influx induced by the ionophore monensin on hepatocellular carcinoma models was compared with that on hepatocytes and normal tissues
|
|---|---|
| Study Description |
The effect of monensin treatment in vitro and in vivo in hepatocellular carcinoma models
|
| Study Type |
Preclinical
|
| Study Subtype |
In vivo ex vivo
|
Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Boosting intracellular sodium selectively kills hepatocarcinoma cells and induces hepatocellular carcinoma tumor shrinkage in mice.
|
| Paper Authors |
Nausicaa Clemente1, Simona Baroni2, Simone Fiorilla1, Francesco Tasso1, Simone Reano3, Chiara Borsotti1, Maria Rosaria Ruggiero2, Elisa Alchera4, Marco Corrazzari5, Gillian Walker1, Antonia Follenzi1, Simonetta Geninatti Crich2 and Rita Carini1
|
| Affiliation |
Department of Health Science University del Piemonte Orientale, Novara, Italy; Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Department of Translational Medicine, Unit of Muscle Biology, Università del Piemonte Orientale, Novara, Italy; Division of Experimental Oncology Unit of Urology, URI, IRCCS, Ospedale San Raffaele, Milan, Italy; Department of Health Science and Interdisciplinary Research Center of Autoimmune Disease (IRCAD), Università del Piemonte Orientale, Novara, Italy.
|
| Paper Journal |
Communications Biology
|
| Paper Year |
2023
|
| Paper DOI |
Under pubblication
|
| Open Access |
Yes
|
Study Component
| Multi Modality Images |
No
|
|---|---|
| Imaging Modality |
MRI
|
| Imaging Sub Modality |
Surface coil 1H T2w, 23 Na Gradient-echo FLASH
|
| Radiation |
No
|
| Imaging Coverage |
Sub-cutaneous tumor in the back
|
| Imaging Target |
Anatomy, Sodium tissue content
|
Dataset Information
| Institution |
UniTO
|
|---|---|
| PI |
S. Genibatti Crich
|
| Country Of Institution |
Italy
|
| Imaging Facility |
Laboratory of molecular imaging for small animal
|
| Euro Bio Imaging Node |
MMMI
|
| Country Of Imaging Facility |
Italy
|
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
2
|
|---|---|
| Types Of Groups |
Control and monensin (16 mg Kg) treated groups
|
| Overall Sample Size |
8
|
| Disease Model |
Cancer
|
| Organ Or Tissue |
Hepatocellular carcinoma
|
| Sample Size For Each Group |
4,4
|
| Randomization |
Mice were randomly divided into treted and control group
|
| Blinding |
No
|
| Outcome Measures |
Sodium tissue content
|
| Statistical Methods |
T-test
|
| Species |
Mice
|
| Strain |
NSG (NOD.Cg-Prkdcscid Il2rgtm1 Wjl SzJ)
|
| Immune Status |
Extremely immunodeficient
|
| Sex |
Male
|
| Age |
Six-to-eight-week-old
|
| Weight |
About 25 g
|
| Genotype |
Prkdcscid Il2rgtm1 Wjl
|
| Source Of Animals |
The Jackson lab
|
| Registry Number Of Animal Authorization |
851 2020-PR
|
Experimental Procedures
| Pharmacological Procedures Intervention And Control |
Yes
|
|---|---|
| Pharmacological Drug |
Monensin
|
| Company |
Merck
|
| Formulation |
Solution from powder
|
| Drug Dose |
16 mg Kg
|
| Volume |
About 0.1 ml
|
| Site Or Route Of Administration |
Intraperitoneal
|
| Frequency Of Administration |
Daily
|
| Vehicle Or Carrier Solution Formulation |
Dilusion of the stock solution (DMSO 2-Hydroxypropyl)-b-cyclodextrin 10%) in saline
|
| Blood Sampling |
No
|
| Surgical Procedures Including Sham Surgery |
No
|
| Target Organ Tissue |
Tumor
|
| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia Type |
Intramuscolar
|
| Anesthesia Drugs |
Zoletil Rompun
|
| Anesthesia Dose |
20 mg kg tiletamine zolazepam (Zoletil 100; Virbac, Milan, Italy) and 5 mg kg xylazine (Rompun; Bayer, Milan, Italy).
|
| Euthanasia |
Yes
|
| Method |
Cervival dislocation
|
| Histology |
No
|
| Frequency Of Imaging |
Daily every 2 days
|
| Timing Of Imaging |
2 h after treatment
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
No
|
| Cell Line |
Hepa Hepa1c1c7 (C1C7) hepatoma cells
|
| Provenance |
ECACC (Salisbury, UK)
|
| Modified Cell Line |
No
|
| Cell Injection Route |
Subcutaneous
|
| Number Of Cells |
1x10^6
|
Image Acquisition
| Instrument Vendor |
Bruker
|
|---|---|
| Instrument Type |
Pharmascan 70 16, equipped with a 1H 23 Na transmit-receive surface coil (20 mm diameter)
|
| Instrument Specifics |
7 T
|
| Image Acquisition Parameters |
Proton T2-weighted images were collected using a Turbo RARE pulse sequence (FOV 30x30, MTX 128x128, slice thickness 3 mm, spatial resolution 0.23 x 0.23 mm2, TE 55 ms, TR 2500 ms, RF 12, average 1). The Na+ gradient-echo FLASH sequence was set with the following parameters: FOV 30x30, MTX 32x32, slice thickness 3 mm, spatial resolution 0.94 x 0.94 mm2, TE 2.05 ms, TR 50 ms, average 548.
|
| Correction |
No
|
Image Data
| Image Type |
Primary image
|
|---|---|
| Format Compression |
Raw; DICOM
|
| Dimensions |
2D
|
| Field Of View |
30x30
|
| Dimension Extents |
1H 128x128, 23 Na 32x32
|
| Size Description |
1H 128x128x3 mm, 23 Na 32x32x3 mm
|
| Image Processing Methods |
Default reconstruction
|
Analyzed Data
| Analysis Result Type |
Numerical
|
|---|---|
| Data Used For Analysis |
ROI, Na standard reference
|
| Analysis Method And Details |
Manual delineation of tumor regions
|
| Status |
Complete
|
| Updated Year |
2023
|