Dataset ID: 14
Dataset Details
Study Design
| Study Design Background |
Evaluation of new type 61 Cu-labelled radiodiagnostic
|
|---|---|
| Study Description |
NAPamide-targeted radiopharmaceutical
|
| Study Type |
Preclinical
|
| Study Subtype |
In vitro, in vivo, ex vivo
|
Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
61 Cu-Labelled radiodiagnostics of melanoma with NAPamide-targeted radiopharmaceutical
|
| Paper Authors |
Kálmán-Szabó I, Bunda Sz, Lihi N, Szaniszló Zs, Szikra D, Péliné Szabó J, Fekete A, Gyuricza B, Szücs D, Papp G, Trencsényi Gy, Kálmán F
|
| Affiliation |
Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, University of Debrecen; Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen; Department of Physical Chemistry,University of Debrecen; ELKH-DE, Mechanisms of Complex Homogeneous and Heterogeneous Chemical Reactions Research Group, Department of Inorganic and Analytical Chemistry
|
| Paper Journal |
International Journal Of Pharmaceutics
|
| Paper Year |
2023
|
| Paper DOI | |
| Open Access |
No
|
Study Component
| Multi Modality Images |
No
|
|---|---|
| Imaging Modality |
PET
|
| Radiation |
Yes
|
| Imaging Coverage |
Body
|
| Imaging Target |
Tumor detection
|
Dataset Information
| Institution |
University of Debrecen
|
|---|---|
| PI |
György Trencsényi
|
| Country Of Institution |
Hungary
|
| Imaging Facility |
Division of Nuclear Medicine and Translational Imaging
|
| Country Of Imaging Facility |
Hungary
|
| Funding |
Yes
|
| Funding Agency |
Hungarian National Research, Development and Innovation Office project, the New National Excellence Program, János Bolyai Research Scholarship of the Hungarian Academy of Sciences, KDP-2021 program of the Ministry for Innovation and Technology from the source of the National Research Development and Innovation Fund.
|
| Grant Number |
FK-134551, ÚNKP-21-5, ÚNKP-22-5, RH 322-8 2022
|
| Dataset Access |
Public
|
| License |
 |
| DUO Data Use Permission |
DUO-0000042
|
| DUO Data Use Modifier |
DUO-0000012
|
| DUO Investigation |
DUO-0000039
|
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
1
|
|---|---|
| Types Of Groups |
Treated
|
| Overall Sample Size |
30
|
| Disease Model |
Cancer
|
| Organ Or Tissue |
Epidermis (melanoma)
|
| Sample Size For Each Group |
30
|
| Randomization |
No
|
| Blinding |
No
|
| Outcome Measures |
Standardized uptake values (SUV)
|
| Statistical Methods |
Two-way ANOVA, Students t-test (two-tailed), Mann-Whitney U test using the Med Calc 18.5 software package (Med Calc Software, Mariakerke, Belgium)
|
| Species |
Mice
|
| Strain |
C57BL 6J
|
| Immune Status |
Immunecompetent
|
| Sex |
Female
|
| Age |
12 weeks old
|
| Weight |
20 g
|
| Source Of Animals |
Charles River Laboratories
|
Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
Yes
|
| Blood Timing |
Once
|
| Surgical Procedures Including Sham Surgery |
No (only tumor injection)
|
| Target Organ Tissue |
Epidermis
|
| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia Type |
Gas
|
| Anesthesia Drugs |
Forane
|
| Anesthesia Dose |
1,5% in 400ml min oxygen
|
| Monitoring Regime |
Respiratoy rate, cardiac rate
|
| Euthanasia |
Yes
|
| Method |
Euthanized with 5 % Forane
|
| Histology |
No
|
| Frequency Of Imaging |
One-day experiment (after 30-, 60-, 90- and 180-min incubation time)
|
| Timing Of Imaging |
8 ± 1 days after tumor cell inoculation
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
|
| Contrast Agent Chemical Drug |
[61 Cu]Cu-KFTG-NAPamide
|
| Contrast Agent Dose |
10.3 ± 0.3 MBq
|
| Injection Volume |
Depends on the radioactive concentration
|
| Route Of Administration |
Intravenous
|
| Cell Line |
B16-F10
|
| Provenance |
American Type Culture Collection (ATCC)
|
| Modified Cell Line |
No
|
| Cell Injection Route |
Subcutaneous
|
| Number Of Cells |
1x10^6
|
| Housing Room |
SPF
|
| Dietary Intervention |
No
|
| Respiration Rate |
No
|
| Body Tempurature Etc |
No
|
| Food Intake Measured |
No
|
Image Acquisition
| Instrument Vendor |
University of Debrecen (own development)
|
|---|---|
| Instrument Type |
MiniPET-II imaging device
|
| Instrument Specifics |
Axial and radial FOV are 48 mm and 106 mm, respectively
|
| Correction |
Decay correction, random correction
|
| Raw Data |
Yes
|
Image Data
| Image Type |
Processed image
|
|---|---|
| Image Scale |
SUV
|
| Format Compression |
DICOM, MINC, NifTI-1
|
| Dimensions |
2D
|
| Field Of View |
Axial FOV 48mm, Ring diameter 211mm, Transaxial FOV 100mm
|
| Dimension Extents |
Volume in pixels: 296, 296, 35
|
| Size Description |
Physical size of image volume in x,y,z & units (pull-down) 296*0,2694, 296*0,2694,35*1,347
|
| PI xel Voxel Size Description |
1.52 x 1.52 x 10 mm3
|
| Image Processing Methods |
Image reconstruction, smoothing
|
| Image Reconstruction Algorithm |
2D 3D FBP, ML-EM, OSEM, ART and MAP
|
| Quality Control |
Ramlak filter with a cut-off frequency of 0.9
|
| Image Smoothing Or Filtering Algorithm |
Brain Cad
|
| Ai Enhanced Algorithm |
Yes
|
| Corrections |
Decay correction
|
Analyzed Data
| Data Used For Analysis |
SUV
|
|---|---|
| Status |
Complete
|
| Updated Year |
2024
|