Dataset ID: 15
Dataset Details
Study Design
| Study Design Background |
Development of metrics for improving tumor pH quantification in presence of fat
|
|---|---|
| Study Description |
CEST tumor pH fat correction
|
| Study Type |
Preclinical
|
| Study Subtype |
In vitro in vivo
|
Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Development and Validation of Four Different Methods to Improve MRI-CEST Tumor pH Mapping in Presence of Fat
|
| Paper Authors |
Gammaraccio F, Villano D, Irrera P, Anemone AA, Carella A, Corrado A, Longo DL
|
| Affiliation |
Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy; Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy;
|
| Paper Journal |
Journal of Imaging
|
| Paper Year |
2024
|
| Paper DOI | |
| Open Access |
Yes
|
Study Component
| Multi Modality Images |
No
|
|---|---|
| Imaging Modality |
MRI
|
| Imaging Sub Modality |
CEST
|
| Radiation |
No
|
| Imaging Coverage |
Abdomen
|
| Imaging Target |
Tumor
|
Dataset Information
| Institution |
CNR-IBB
|
|---|---|
| PI |
Dario Longo
|
| Country Of Institution |
Italy
|
| Imaging Facility |
CNR-IBB Torino
|
| Euro Bio Imaging Node |
MMMI
|
| Country Of Imaging Facility |
Italy
|
| Link To Dataset | |
| Funding |
Yes
|
| Funding Agency |
Associazione Italiana Ricerca sul Cancer (AIRC)
|
| Grant Number |
MFAG 2017 #20153
|
| Dataset Access |
Public
|
| License |
 |
| DUO Data Use Permission |
DUO-0000042
|
| DUO Data Use Modifier |
DUO-0000019, DUO-0000046
|
| DUO Investigation |
DUO-0000037, DUO-0000040, DUO-0000031
|
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
2
|
|---|---|
| Types Of Groups |
Low fat, high fat
|
| Overall Sample Size |
2
|
| Disease Model | |
| Organ Or Tissue |
Mammary gland
|
| Sample Size For Each Group |
1.1
|
| Randomization |
No
|
| Procedures To Keep Treatments Blind |
No
|
| Outcome Measures |
CEST contrast, tumor pH
|
| Species |
Mice
|
| Strain |
Balb C, MMTV-PyMT
|
| Immune Status |
Immunecompetent
|
| Sex |
Female, female
|
| Age |
7 weeks, 8 weeks
|
| Weight |
25 grams
|
| Genotype |
Heterozygous
|
| Genetic Manipulation |
Transgenic
|
| Source Of Animals |
Charles River Laboratories Italia srl, Calco Italy; The Jackson Laboratory, Bar Harbor, ME, USA
|
Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
No
|
| Surgical Procedures Including Sham Surgery |
No
|
| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia Type |
Intramuscular
|
| Duration |
40
|
| Anesthesia Drugs |
Xylazine (Rompun, Bayer, Italy), tiletamine zolazepam (Zoletil 100, Virbac, Italy)
|
| Anesthesia Dose |
5 mg kg, 20 mg kg
|
| Euthanasia |
Yes
|
| Method |
Cervical dislocation
|
| Histology |
No
|
| Frequency Of Imaging |
Single scan
|
| Overall Scan Length |
40 minutes
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
|
| Contrast Agent Commercial Drug |
Isovue
|
| Contrast Agent Chemical Drug |
Iopamidol
|
| Contrast Agent Dose |
4 g kg
|
| Injection Volume |
0.25
|
| Route Of Administration |
Intravenous
|
| Cell Line |
4T1
|
| Provenance |
Molecular Biotechnology Center Cell Factory
|
| Modified Cell Line |
No
|
| Cell Injection Route |
Subcutaneous
|
| Number Of Cells |
1000000
|
| Housing Room |
Not SPF
|
| Dietary Intervention |
No
|
| Respiration Rate |
No
|
| Body Tempurature Etc |
No
|
| Food Intake Measured |
No
|
Image Acquisition
| Instrument Vendor |
Bruker
|
|---|---|
| Instrument Type |
Microimaging
|
| Instrument Specifics |
7T
|
| Raw Data |
Yes
|
| QA/QC |
No
|
Image Data
| Image Type |
Primary image processed image
|
|---|---|
| Image Scale |
Signal intensity
|
| Format Compression |
DICOM
|
| Dimensions |
4D (3D + frequencies)
|
| Overall Number Of Images |
16
|
| Field Of View |
30x30x1.5 mm
|
| Dimension Extents |
128x128x8
|
| Size Description |
30x30x12 mm
|
| PI xel Voxel Size Description |
0,234x0,234x1,5 mm
|
| Image Processing Methods |
Default reconstruction
|
| Quality Control |
No
|
| Image Registration Algorithm |
No
|
| Ai Enhanced Algorithm |
Yes
|
| QC Info |
R2 calculation on raw CEST data
|
Analyzed Data
| Analysis Result Type |
Numerical analysis
|
|---|---|
| Data Used For Analysis |
PH maps
|
| Analysis Method And Details |
Manual delineation of tumor regions (ROI), MATLAB
|
| File Format Of Result File Csv Json Txt Xlsx |
Jpg, txt, mat
|
| Status |
Complete
|
| Updated Year |
2024
|