Dataset ID: 17
Dataset Details
Study Design
| Study Design Background |
The study provides evidence that SYRO plus MET has a synergistic effect on tumor growth inhibition in both 4T1 and TS A experimental models
|
|---|---|
| Study Description |
SYRO plus MET, this radiotracer could be potentially used as a biomarker for the early detection of therapy response
|
| Study Type |
Preclinical
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| Study Subtype |
In vivo, ex vivo
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Publication
| Paper Linked |
Yes
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|---|---|
| Paper Title |
Evaluating [18F]FDG and [18F]FLT Radiotracers as Biomarkers of Response for Combined Therapy Outcome in Triple-Negative and Estrogen-Receptor-Positive Breast Cancer models
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| Paper Authors |
Rainone P, Valtorta S, Villa C, Todde S, Cadamuro M, Bertoli G, Conconi D, Lavitrano M, Moresco RM
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| Affiliation |
University of Milano - Bicocca
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| Paper Journal |
International Journal of Molecular Sciences
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| Paper Year |
2023
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| Paper DOI | |
| Open Access |
Yes
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Study Component
| Multi Modality Images |
Yes
|
|---|---|
| Imaging Modality |
PET CT
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| Radiation |
Yes
|
| Imaging Coverage |
Whole body
|
| Imaging Target |
Tumor
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Dataset Information
| Institution |
University of Milano - Bicocca
|
|---|---|
| PI |
Prof. Moresco Rosa Maria
|
| Country Of Institution |
Italy
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| Imaging Facility |
The Laboratory of Imaging for small animals of IBSBC-CNR
|
| Euro Bio Imaging Node |
MMMI
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| Country Of Imaging Facility |
Italy
|
| Funding |
Yes
|
| Funding Agency |
MOLIM ONCOBRAIN LAB—Innovative methods of molecular imaging for the study of oncological and neurodegenerative diseases, AIRC
|
| Grant Number |
2017-NAZ-0146 A, grant n.21635
|
| Dataset Access |
Limited
|
| DUO Data Use Permission |
DUO-0000042
|
| DUO Data Use Modifier |
DUO-0000046
|
| DUO Investigation |
DUO-0000040
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| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
6
|
|---|---|
| Types Of Groups |
Control, cisplatin, MET, cisplatin plus MET, SYRO, and SYRO plus MET
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| Overall Sample Size |
57
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| Disease Model |
Breast cancer
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| Organ Or Tissue |
Mammary gland
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| Sample Size For Each Group |
5 o 4
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| Randomization |
Yes, when tumors were palpable, the animals inoculated with 4T1 or TS A were randomly assigned
|
| Blinding |
No
|
| Statistical Methods |
Student’s t-test
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| Species |
Mice
|
| Strain |
Balb c
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| Immune Status |
Immunecompetent
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| Sex |
Female
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| Age |
7-8 weeks
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| Age At Start Experiment |
8 weeks
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| Age At Scanning Experiment S |
56 and 63 days
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| Weight |
24-26 g
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| Source Of Animals |
Charles River Laboratories, Italy
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Experimental Procedures
| Pharmacological Procedures Intervention And Control |
Yes
|
|---|---|
| Pharmacological Drug |
Metformin, syrosingopine, cisplatin
|
| Company |
Sigma Aldrich
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| Drug Dose |
MET 250 mg kg, SYRO 7.5 mg kg, cisplatin 3 mg kg
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| Site Or Route Of Administration |
Intraperitoneal
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| Frequency Of Administration |
MET daily, SYRO three times for week, cisplatin twice a week
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| Vehicle Or Carrier Solution Formulation |
All drugs dissolved in saline, except SYRO that was dissolved in DMSO (10% in sterile water)
|
| Blood Sampling |
No
|
| Surgical Procedures Including Sham Surgery |
No
|
| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia For Imaging |
Yes
|
| Anesthesia Type |
Gas
|
| Duration |
20
|
| Anesthesia Drugs |
Isoflurane
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| Anesthesia Dose |
2%
|
| Euthanasia |
Yes
|
| Method |
Anesthesia
|
| Histology |
Yes
|
| Tissues Collected Post Euthanasia |
Tumor
|
| Timing Of Collection |
End of study
|
| Imaging |
Yes
|
| Frequency Of Imaging |
Single scan
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| Timing Of Imaging |
T0 and after 7 days of the treatment
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
|
| Contrast Agent Commercial Drug |
[18F]FDG, [18F]FLT
|
| Contrast Agent Chemical Drug |
18F-fluorodeoxyglucose (FDG), 18F-fluorothymidine (FLT)
|
| Contrast Agent Dose |
4.67 ± 0.3 MBq
|
| Injection Volume |
0.05 - 0.1 ml
|
| Route Of Administration |
Intravenous
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| Cell Lines |
Yes
|
| Cell Line |
4T1, TS A
|
| Provenance |
TS A (Sigma-Aldrich Inc., St. Louis, MO, USA), 4T1 (ATCC)
|
Image Acquisition
| Instrument Vendor |
Molecubes
|
|---|---|
| Instrument Type |
Β-cube, X-cube
|
| Instrument Specifics |
FOV size for PET 130X72 mm, FOV size for CT 37X65 mm
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| Image Acquisition Parameters |
CT scan (exam duration: 4 min, X-Ray beam duration: 90 s, k Vp: 40, current:400 A, rotation time: 60 s, and angular views: 960), PET scanner for a 20 min static acquisition
|
| Correction |
Decay correction (PET)
|
| Raw Data |
Yes
|
Image Data
| Image Type |
Processed images (reconstructed PET and CT data)
|
|---|---|
| Image Scale |
SUV for PET
|
| Format Compression |
DICOM
|
| Dimensions |
3D
|
| Overall Number Of Images |
36
|
| Field Of View |
Abdominal
|
| Dimension Extents |
192x192x384
|
| PI xel Voxel Size Description |
0.4x0.4x0.4
|
| Image Processing Methods |
Image reconstruction
|
| Image Reconstruction Algorithm |
OSEM
|
| Ai Enhanced Algorithm |
Yes
|
Analyzed Data
| Data Used For Analysis |
SUV, volume measurements
|
|---|---|
| Status |
Complete
|
| Updated Year |
2024
|