Dataset ID: 18
Dataset Details
Study Design
| Study Design Background |
Evaluation of new type 213 Bi-labelled alpha-melanocyte stimulating hormone peptide analogues in preclinical melanoma model
|
|---|---|
| Study Description |
213 Bi-labelled DOTA-conjugated alpha-melanocyte stimulating hormone peptide analogues
|
| Study Type |
Preclinical
|
| Study Subtype |
In vivo, ex vivo
|
Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Evaluation of the therapeutic efficacy of 213 Bi-labelled DOTA-conjugated alpha-melanocyte stimulating hormone peptide analogues in melanocortin-1 receptor positive preclinical melanoma model
|
| Paper Authors |
Csikos Csaba, Képes Zita, Fekete Anikó Anikó, Vágner Adrienn, Nagy Gábor, Gyuricza Barbara, Arató Viktória, Kárpáti Levente, Mándity István, Bruchertseifer Frank, Halmos Gábor Halmos, Szikra Dezső, Trencsényi György
|
| Affiliation |
Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen; Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen; Scanomed Ltd., Doctoral School of Chemistry, Faculty of Science and Technology, University of Debrecen; Doctoral School of Pharmaceutical Sciences, University of Debrecen; Department of Organic Chemistry, Faculty of Pharmacy, Semmelweis University; Artificial Transporters Research Group, Research Centre for Natural Sciences; European Commission, Joint Research Centre (JRC); Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen
|
| Paper Journal |
International Journal of Pharmaceutics
|
| Paper Year |
2023
|
| Paper DOI | |
| Open Access |
No
|
Study Component
| Multi Modality Images |
Yes
|
|---|---|
| Imaging Modality |
PET MRI
|
| Imaging Sub Modality |
T2w
|
| Radiation |
Yes
|
| Imaging Coverage |
Whole body
|
| Imaging Target |
Receptor expression
|
Dataset Information
| Institution |
University of Debrecen
|
|---|---|
| PI |
Trencsényi György
|
| Country Of Institution |
Hungary
|
| Imaging Facility |
Division of Nuclear Medicine and Translational Imaging
|
| Country Of Imaging Facility |
Hungary
|
| Funding |
Yes
|
| Funding Agency |
Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund
|
| Grant Number |
TKP2020-NKA-04
|
| Dataset Access |
Public
|
| License |
 |
| DUO Data Use Permission |
DUO-0000042
|
| DUO Data Use Modifier |
DUO-0000012
|
| DUO Investigation |
DUO-0000039
|
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
3
|
|---|---|
| Types Of Groups |
Untreated, treated1, treated2
|
| Overall Sample Size |
20
|
| Disease Model |
Cancer
|
| Organ Or Tissue |
Epidermis
|
| Sample Size For Each Group |
Untreated 5, treated1 9, treated2 6
|
| Randomization |
No
|
| Blinding |
No
|
| Species |
Mice
|
| Strain |
C57BL 6J
|
| Immune Status |
Immunecompetent
|
| Sex |
Male and female
|
| Age |
16 weeks old
|
| Weight |
20 ± 0,3 grams
|
| Source Of Animals |
Charles River Laboratories
|
Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
Yes
|
| Blood Timing |
Once
|
| Surgical Procedures Including Sham Surgery |
No (only tumor injection)
|
| Target Organ Tissue |
Epidermis
|
| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia For Imaging |
Yes
|
| Anesthesia Type |
Gas
|
| Anesthesia Drugs |
Forane
|
| Anesthesia Dose |
1,5% in 400ml min oxygen
|
| Monitoring Regime |
Respiratoy rate, cardiac rate
|
| Euthanasia |
Yes
|
| Method |
Euthanized with 5 % Forane
|
| Histology |
Yes
|
| Tissues Collected Post Euthanasia |
Epidermis
|
| Timing Of Collection |
At euthanasia
|
| Imaging |
Yes
|
| Frequency Of Imaging |
One-day experiment
|
| Timing Of Imaging |
10 ± 1 days after subcutaneous tumor cell inoculation
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
|
| Contrast Agent Chemical Drug |
[68 Ga]Ga-DOTA-HOLDamide, [68 Ga]Ga-DOTA-NAPamide
|
| Contrast Agent Dose |
15.6 ± 1.2 MBq
|
| Injection Volume |
Depends on the radioactive concentration
|
| Route Of Administration |
Intravenous
|
| Cell Lines |
Yes
|
| Cell Line |
B16-F10
|
| Provenance |
American Type Culture Collection (ATCC)
|
| Modified Cell Line |
No
|
| Cell Injection Route |
Subcutaneous
|
| Number Of Cells |
2x10^6
|
| Housing Room |
SPF
|
Image Acquisition
| Instrument Vendor |
Mediso Ltd.
|
|---|---|
| Instrument Type |
Nano Scan PET MRI
|
| Instrument Specifics |
3T, FOV: 32 mm × 32 mm
|
| Correction |
Decay correction, attenuation correction
|
| Raw Data |
Yes
|
Image Data
| Image Type |
Processed image
|
|---|---|
| Image Scale |
SUV
|
| Format Compression |
DICOM
|
| Dimensions |
2D,3D
|
| Overall Number Of Images |
70
|
| Field Of View |
Bore size: 16 cm, Transaxial FOV: 12 cm, Axial FOV: 10 cm
|
| Dimension Extents |
296, 296, 35
|
| Size Description |
296*0,2694, 296*0,2694,35*1,347
|
| PI xel Voxel Size Description |
1.12 mm×1.12 mm×13 mm
|
| Image Processing Methods |
Reconstruction, smoothing
|
| Image Reconstruction Algorithm |
OSEM-LOR Spatial resolution with Tera-Tomo (3D OSEM): 0.7 mm, Spatial Resolution with FBP (NEMA): 1.25 mm
|
| Quality Control |
Smoothing factor of 0.02
|
| Image Smoothing Or Filtering Algorithm |
Brain Cad
|
| Image Registration Algorithm |
AI denoising
|
| Ai Enhanced Algorithm |
Yes
|
Analyzed Data
| Data Used For Analysis |
SUV
|
|---|---|
| Status |
Complete
|
| Updated Year |
2024
|