Dataset ID: 19
Dataset Details
Study Design
| Study Design Background |
This preclinical study is aimed at developing and evaluating a novel radiotracer for HER2-targeted therapy selection and monitoring. The research focuses on the design, synthesis and characterization of a new Mn(II) chelator, BPPA, which is conjugated to trastuzumab and labeled with [52 Mn]Mn isotope
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|---|---|
| Study Description |
Evaluation of [52 Mn]Mn-BPPA-Trastuzumab
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| Study Type |
Preclinical
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| Study Subtype |
In vivo, ex vivo
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Publication
| Paper Linked |
No
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|---|---|
| Paper Title |
[52 Mn]Mn-BPPA-Trastuzumab: A Promising HER2-Specific PET Radiotracer
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| Paper Authors |
Toàn Ngô Minh, Vágner Adrienn, Nagy Gábor, Ország Gábor, Nagy Tamás, Csikos Csaba, Váradi Balázs, Sajtos Gergo, Kapus István, Szoboszlai Zoltán, Szikra Dezso, Trencsényi György, Tircsó Gyula, Garai Ildiko
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| Affiliation |
Medical Imaging Clinic, Clinical Centre, University of Debrecen; Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen; Scanomed Ltd; Department of Physical Chemistry, Institute of Chemistry, Faculty of Science and Technology, University of Debrecen; Doctoral School of Chemistry, Faculty of Science and Technology, University of Debrecen
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| Paper Journal |
Journal of Medicinal Chemistry
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| Paper Year |
2024
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| Paper DOI | |
| Open Access |
No
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Study Component
| Multi Modality Images |
Yes
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|---|---|
| Imaging Modality |
PET MRI
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| Imaging Sub Modality |
T2w
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| Radiation |
Yes
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| Imaging Coverage |
Whole body
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| Imaging Target |
Receptor expression
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Dataset Information
| Institution |
University of Debrecen
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|---|---|
| PI |
Ildikó Garai
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| Co PI |
Gyula Tircsó
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| Country Of Institution |
Hungary
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| Country Of Imaging Facility |
Hungary
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| Funding |
Yes
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| Funding Agency |
Gedeon Richter Ph.D. Fellowship; Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund
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| Grant Number |
K-134694
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| Dataset Access |
Public
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| License |
 |
| DUO Data Use Permission |
DUO-0000042
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| DUO Data Use Modifier |
DUO-0000012
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| DUO Investigation |
DUO-0000039
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| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
7
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|---|---|
| Types Of Groups |
Untreated1, untreated2, untreated3, treated1, treated2, treated3, treated4
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| Overall Sample Size |
20
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| Disease Model |
Cancer
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| Organ Or Tissue |
Mammary gland
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| Sample Size For Each Group |
Untreated 8, treated1 3, treated2 3, treated3 3, treated4 3
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| Randomization |
No
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| Blinding |
No
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| Species |
Mice
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| Strain |
Balb c and SCID
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| Immune Status |
Immunecompetent, immunedefficient
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| Sex |
Female
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| Age |
12 weeks old
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| Weight |
19.5 ± 0.5 grams
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| Source Of Animals |
Charles River Laboratories
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Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
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|---|---|
| Blood Sampling |
Yes
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| Blood Timing |
Once
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| Surgical Procedures Including Sham Surgery |
No (only tumor injection)
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| Target Organ Tissue |
Mammary gland
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| Pathogen Infection Intervention And Control |
No
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| Analgesic Plan To Relieve Pain Suffering And Distress |
No
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| Anesthesia For Imaging |
Yes
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| Anesthesia Type |
Gas
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| Anesthesia Drugs |
Forane
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| Anesthesia Dose |
1,5% in 400ml min oxygen
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| Monitoring Regime |
Respiratoy rate, cardiac rate
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| Euthanasia |
Yes
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| Method |
Euthanized with 5 % Forane
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| Histology |
Yes
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| Tissues Collected Post Euthanasia |
Mammary gland tissue
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| Timing Of Collection |
At euthanasia
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| Imaging |
Yes
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| Frequency Of Imaging |
One-day experiment
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| Timing Of Imaging |
7 days after the tumor cell inoculation
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
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| Contrast Agent Chemical Drug |
[52 Mn]Mn-DOTA-GA, [52 Mn]Mn-BPPA, [52 Mn]Mn Cl2
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| Contrast Agent Dose |
2.4±0.4 MBq
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| Injection Volume |
Depends on the radioactive concentration
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| Route Of Administration |
Intravenous
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| Cell Lines |
Yes
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| Cell Line |
4T1, MDA-MB-HER2+
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| Provenance |
American Type Culture Collection (ATCC)
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| Housing Room |
SPF
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Image Acquisition
| Instrument Vendor |
Mediso Ltd.
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|---|---|
| Instrument Type |
Nano Scan PET MRI
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| Instrument Specifics |
3T, FOV: 32 mm × 32 mm
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| Correction |
Decay correction, attenuation correction
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| Raw Data |
Yes
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Image Data
| Image Type |
Processed image
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|---|---|
| Image Scale |
SUV
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| Format Compression |
DICOM
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| Dimensions |
2D, 3D
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| Field Of View |
Bore size: 16 cm, Transaxial FOV: 12 cm, Axial FOV: 10 cm
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| Dimension Extents |
296, 296, 35
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| Size Description |
296*0, 2694, 296*0, 2694, 35*1, 347
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| PI xel Voxel Size Description |
1.12 mm×1.12 mm×13 mm
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| Image Processing Methods |
Reconstruction, smoothing
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| Image Reconstruction Algorithm |
OSEM-LOR Spatial resolution with Tera-Tomo (3D OSEM): 0.7 mm, Spatial Resolution with FBP (NEMA): 1.25 mm
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| Quality Control |
Smoothing factor of 0.02
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| Image Smoothing Or Filtering Algorithm |
Brain Cad
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| Image Registration Algorithm |
AI denoising
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| Ai Enhanced Algorithm |
Yes
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Analyzed Data
| Data Used For Analysis |
SUV
|
|---|---|
| Status |
Complete
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| Updated Year |
2025
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