Dataset ID: 22
Dataset Details
Study Design
| Study Design Background |
Sudy of rare disease (aceruplasmonemia) using Cp KO mouse model to highlight gender differences in disease behaviour in view of a possible replacement therapy. Labeling of Ceruloplasmine (Cp) with Cu-64 to follow its in vivo distribution in the model. Study of inflammation.
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|---|---|
| Study Description |
Ceruloplasmin administration in the preclinical mouse model of aceruloplasminemia reveals a sex-related variation in biodistribution
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| Study Type |
Preclinical
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| Study Subtype |
In vivo and post mortem
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Publication
| Paper Linked |
Yes
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|---|---|
| Paper Title |
Ceruloplasmin administration in the preclinical mouse model of aceruloplasminemia reveals a sex-related variation in biodistribution
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| Paper Authors |
Sara Belloli, Cristina Monterisi, Paolo Rainone, AngelaColiva, Alan Zanardi, Antonio Conti, Andrea Caricasole, Rosa Maria Moresco & Massimo Alessio
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| Affiliation |
1. Nuclear Medicine and PET Cyclotron Unit, IRCCS-Ospedale San Raffaele, Milano, Italy. 2. Institute of Bioimaging and Complex Biological Systems, CNR, Segrate, MI, Italy. 3. Medicine and Surgery Department, University of Milano - Bicocca, Monza, MB, Italy. 4. Proteome Biochemistry, COSR-Center for Omics Sciences, IRCCS-Ospedale San Raffaele, Milano, Italy. 5. Research and Innovation, Kedrion S.p.A., Loc. Bolognana, Gallicano, LU, Italy. 6. Proteome Biochemistry, COSR-Center for Omics Sciences, IRCCS-Ospedale San Raffaele, Milano, Italy.
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| Paper Journal |
Communications biology
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| Paper Year |
2025
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| Paper DOI | |
| Open Access |
Yes
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Study Component
| Multi Modality Images |
No
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|---|---|
| Imaging Modality |
PET/CT
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| Radiation |
Yes
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| Imaging Coverage |
Whole body
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| Imaging Target |
Copper transporter, TSPO receptor
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Dataset Information
| Institution |
IBSBC-CNR
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|---|---|
| PI |
Massimo Alessio
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| Co PI |
Rosa Maria Moresco
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| Country Of Institution |
Italy
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| Imaging Facility |
Laboratory of Preclinical Molecular Imaging
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| Euro Bio Imaging Node |
Multi-Modal Molecular Imaging Italian node (MMMI)
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| Country Of Imaging Facility |
Italy
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| Funding |
Yes
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| Funding Agency |
Ricerca Finalizzata 2018, Italian Ministry ofHealth (RF-2018-12366471), Eu-BI MMMI FOE 2021, PNRR See Life Project IR0000023
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| Dataset Access |
Public
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| License |
 |
| DUO Data Use Permission | |
| DUO Data Use Modifier | |
| DUO Investigation | |
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
8
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|---|---|
| Types Of Groups |
Female adult wt, female wt aged, female adult KO, female aged KO, male adult wt, male wt aged, male adult KO, male aged KO
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| Overall Sample Size |
48
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| Main Disease Category |
Disease of metabolism
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| Specific Disease Model |
Rare disease with neurological signs and inflammation
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| Organ Or Tissue |
Brain and liver
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| Sample Size For Each Group |
6 per each group
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| Power Calculation |
G*Power
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| Randomization |
No
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| Blinding |
No
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| Species |
Mice
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| Strain |
C57BL/6
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| Immune Status |
Immunocompetent
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| Sex |
Female and male
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| Age |
Adult and aged
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| Age At Start Experiment |
6 months
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| Weight |
40 g
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| Genotype |
Homozygous
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| Genetic Manipulation |
Cp KO
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| Gene |
Ceruloplasmine
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Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
Yes
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| Blood Sampling Method |
Cardiac puncture before sacrifice
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| Blood Sample Volume |
500 microliter
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| Blood Timing |
One
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| Surgical Procedures Including Sham Surgery |
No
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| Target Organ Tissue |
Brain, eyes, liver, small intestine, lung, kidney, heart and chest muscle
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| Pathogen Infection Intervention And Control |
No
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| Analgesic Plan To Relieve Pain Suffering And Distress |
No
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| Anesthesia For Imaging |
Yes
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| Anesthesia Type |
Gas
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| Duration |
30 minutes
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| Anesthesia Drugs |
Isoflurane
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| Euthanasia |
Yes
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| Method |
Cardiac puncture and cervical dislocation
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| Histology |
Yes
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| Tissues Collected Post Euthanasia |
Brain, eyes, liver, small intestine, lung, kidney, heart and chest muscle
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| Timing Of Collection |
End of imaging
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| Imaging |
Yes
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| Frequency Of Imaging |
One per group
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| Timing Of Imaging |
Selected age of the Cp KO animals
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| Overall Scan Length |
30 minutes
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
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| Contrast Agent Commercial Drug |
[64Cu]-Ceruloplasmine; [18F]-VC701
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| Contrast Agent Chemical Drug |
[64Cu]-Ceruloplasmine; [18F]-3-Fluoromethyl-N-methyl-4-phenyl-N-(phenylmethyl) quinoline-2-carboxamide
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| Contrast Agent Dose |
5.12 ± 0.25 MBq; 1.91 ± 0.27MBq
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| Injection Volume |
50ul
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| Route Of Administration |
Intravenous
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| Cell Lines |
No
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Image Acquisition
| Instrument Vendor |
Molecubes
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|---|---|
| Instrument Type |
β-cube, X-cube
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| Instrument Specifics |
PET- FOV of 72x130 mm; CT- FOV of 64x40 mm
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| Image Acquisition Parameters |
PET- 511 KeV, 30% energy resolution; CT- 40 kVp, 400 μA (0.8 mm Al filtration)
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| Correction |
PET- isotope decay and random coincidences correction; CT- ring artifact correction
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| Raw Data |
Yes
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| QA/QC |
Yes
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Image Data
| Image Type |
Reconstructed and quantified images
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|---|---|
| Image Scale |
KBq/cc for PET and HU for CT
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| Format Compression |
Raw, DICOM and NIFTI
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| Dimensions |
2D/3D
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| Overall Number Of Images |
1 dataset per animal per imaging technique per time point
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| Field Of View |
Max FOV for PET and CT
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| Dimension Extents |
PET/CT- 192x192x384
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| Size Description |
PET/CT- 76.8 x 76.8 x 154 mm
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| PI xel Voxel Size Description |
PET/CT- 400 µm isometric
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| Image Processing Methods |
CT-based normalization
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| Image Reconstruction Algorithm |
PET- OSEM (30 iterations); CT: ISRA (iterative)
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| Quality Control |
No
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Analyzed Data
| Data Used For Analysis |
SUVmean
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|---|---|
| Updated Year |
2025
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