Dataset ID: 23
Dataset Details
Study Design
| Study Design Background |
This is a preclinical, experimental animal study aimed at investigating tumor progression, metastasis formation, and angiogenesis in a hepatocellular carcinoma (HCC) model. It utilizes PET-based molecular imaging with specific radiopharmaceutical tracers ([18F]FDG, [68Ga]Ga-NOTA-c(NGR), and [68Ga]Ga-NODAGA-[c(RGD)]₂) to characterize metabolic activity and angiogenic marker expression during tumor development.
|
|---|---|
| Study Description |
He/De Metastasis PET Study
|
| Study Type |
Preclinical
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| Study Subtype |
In vivo, ex vivo
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Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Metastasis and angiogenesis: preclinical PET study on hepatocellular carcinoma (He/De) tumor models
|
| Paper Authors |
Barkóczi Alexandra, Képes Zita, Péli-Szabó Judit, Dienes Renáta Adél, Károlyi Péter Kálmán, Papp Tamás, Kálmán-Szabó Ibolya, Sass Tamás, Opposits Gábor, Kertész István, Hajdu István, Trencsényi György, Deák Ádám
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| Affiliation |
Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen Doctoral School of Clinical Medicine, University of Debrecen Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen Division of Radiology and Imaging Science, Department of Medical Imaging, Faculty of Medicine, University of Debrecen Doctoral School of Neuroscience, Faculty of Medicine, University of Debrecen Department of Surgery, Faculty of Medicine, University of Debrecen
|
| Paper Journal |
European Journal of Pharmaceutical Sciences
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| Paper Year |
2025
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| Paper DOI |
doi-10.1016
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| Open Access |
Yes
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Study Component
| Multi Modality Images |
No
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|---|---|
| Imaging Modality |
PET
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| Radiation |
Yes
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| Imaging Coverage |
Abdominal cavity
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| Imaging Target |
Tumor detection
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Dataset Information
| Institution |
University of Debrecen
|
|---|---|
| PI |
Ádám Deák
|
| Co PI |
Alexandra Barkóczi
|
| Country Of Institution |
Hungary
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| Imaging Facility |
Department of Nuclear Medicine and Translational Imaging
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| Euro Bio Imaging Node |
Medical and Preclinical Imaging Hungary
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| Country Of Imaging Facility |
Hungary
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| Funding |
Yes
|
| Funding Agency |
Ministry for Innovation and Technology
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| Grant Number |
KDP-2021
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| Dataset Access |
Public
|
| License |
 |
| DUO Data Use Permission | |
| DUO Data Use Modifier | |
| DUO Investigation | |
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
2
|
|---|---|
| Types Of Groups |
treated1, treated2
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| Overall Sample Size |
30
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| Main Disease Category |
Disease of cellular proliferation
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| Specific Disease Model |
Cancer
|
| Organ Or Tissue |
Liver, kidney
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| Randomization |
No
|
| Blinding |
No
|
| Statistical Methods |
Student's t test (two-tailed), two-way ANOVA, Mann-Whitney rank-sum tests
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| Species |
Rats
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| Strain |
Fischer-344
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| Immune Status |
Immunecompetent
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| Sex |
Male
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| Age |
16 weeks old
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| Weight |
250±20 g
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Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
No
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| Surgical Procedures Including Sham Surgery |
Yes
|
| Description Of The Surgical Procedure |
Subrenal Capsule Assay (SRCA)
|
| Reference To Protocol |
PMID- 19528472
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| Target Organ Tissue |
Subcapsular renal tissue
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| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
Yes
|
| Analgesic Name |
Nurofen
|
| Route |
Oral
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| Analgesic Dose |
10mg/kg
|
| Anesthesia For Imaging |
Yes
|
| Anesthesia Type |
Gas
|
| Anesthesia Drugs |
Forane
|
| Anesthesia Dose |
induction- 3% isoflurane, maintenance- 2% isoflurane combined with 1–2 L/min O2 and 0.8–1 L/min N2O
|
| Euthanasia |
Yes
|
| Method |
Euthanized with 5% Forane
|
| Histology |
No
|
| Imaging |
Yes
|
| Frequency Of Imaging |
one-day experiment
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| Timing Of Imaging |
8 ± 1 days after the subrenal capsule assay
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
|
| Contrast Agent Commercial Drug |
GLUCO-PET
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| Contrast Agent Chemical Drug |
[18F]FDG, [68Ga]Ga-NOTA-c(NGR), [68Ga]Ga-NODAGA-[c(RGD)]2
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| Contrast Agent Dose |
7 MBq
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| Injection Volume |
0.15 mL
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| Route Of Administration |
Intravenous
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| Cell Lines |
Yes
|
| Cell Line |
He/De
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| Provenance |
Chemically induced by the research group
|
| Number Of Cells |
1 × 10^6
|
| Housing Room |
SPF
|
| Dietary Intervention |
No
|
| Respiration Rate |
No
|
| Body Tempurature Etc |
No
|
| Food Intake Measured |
No
|
Image Acquisition
| Instrument Vendor |
University of Debrecen (own development)
|
|---|---|
| Instrument Type |
MiniPET-II imaging device
|
| Instrument Specifics |
Axial and radial FOV are 48 mm and 106 mm, respectively
|
| Image Acquisition Parameters |
350 – 650 keV
|
| Correction |
Decay correction, random correction
|
| Raw Data |
Yes
|
Image Data
| Image Type |
Processed image
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|---|---|
| Image Scale |
SUV
|
| Format Compression |
DICOM, MINC, NifTI-1
|
| Dimensions |
3D
|
| Overall Number Of Images |
30
|
| Field Of View |
48 mm
|
| Dimension Extents |
175 × 175 × 75 voxels
|
| Size Description |
87.5 mm × 87.5 mm × 37.5 mm
|
| PI xel Voxel Size Description |
1.52 x 1.52 x 10 mm3
|
| Image Processing Methods |
Image reconstruction
|
| Image Reconstruction Algorithm |
OSEM (monte Carlo, subsets: 6, iterations: 8)
|
| Quality Control |
Yes
|
| Image Registration Algorithm |
Yes
|
| Ai Enhanced Algorithm |
No
|
Analyzed Data
| Data Used For Analysis |
SUV
|
|---|---|
| Updated Year |
2025
|