Dataset ID: 25
Dataset Details
Study Design
| Study Design Background |
Preclinical study on SOD1-G93A ALS mice assessing skeletal muscle inflammation and mitochondrial dysfunction through [18F]DPA-714 PET/CT imaging, supported by immunohistochemistry, confocal microscopy, and immunofluorescence.
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|---|---|
| Study Description |
This study explores the role of skeletal muscle inflammation and mitochondrial dysfunction in amyotrophic lateral sclerosis (ALS) using the SOD1-G93A mouse model. PET/CT imaging with [18F]DPA-714 was performed to assess TSPO expression in the triceps brachii at different disease stages. Histological analyses, including immunohistochemistry and confocal microscopy, validated PET findings and characterized cellular and molecular changes in muscle tissues.
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| Study Type |
Preclinical
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| Study Subtype |
In vivo
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Publication
| Paper Linked |
Yes
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|---|---|
| Paper Title |
TSPO Expression and [18F]DPA-714 PET/CT Imaging as Pathogenetic and Diagnostic Biomarkers in Symptomatic Stages of Skeletal Muscle Fiber Degeneration in SOD1-G93A ALS Mice
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| Paper Authors |
Anzilotti Serenella, De Iesu Nunzia, Gargiulo Sara, Di Muraglia Noemi, Cicatiello Annunziata Gaetana, Dentice Monica, Panico Mariarosaria, Albanese Sandra, Annunziato Lucio, Salvatore Marco, Pignataro Giuseppe, Pappatà Sabina
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| Affiliation |
1. Department of Human Sciences and Quality of Life Promotion, San Raffaele University, Rome, Italy. 2. IRCCS SYNLAB SDN, Naples, Italy Siena, Italy. 3. Division of Pharmacology, Department of Neuroscience, School of Medicine, University of Naples “Federico II”, Naples, Italy. 4. Department of Clinical Medicine and Surgery, University of Naples “Federico II”, Naples, Italy 5. Institute of Biostructures and Bioimaging, National Research Council, Naples, Italy
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| Paper Journal |
The FASEB Journal
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| Paper Year |
2025
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| Paper DOI | |
| Open Access |
Yes
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Study Component
| Multi Modality Images |
Yes
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|---|---|
| Imaging Modality |
PET/CT
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| Imaging Sub Modality |
18F
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| Radiation |
Yes
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| Imaging Coverage |
Skeletal muscles
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| Imaging Target |
Inflammation
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Dataset Information
| Institution |
IRCCS SYNLAB SDN, Naples, Italy
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|---|---|
| PI |
Sabina Pappatà
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| Country Of Institution |
Italy
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| Imaging Facility |
Prelinical Imaging Facility, Ceinge Biotecnologie Avanzate scarl
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| Country Of Imaging Facility |
Italy
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| Link To Dataset |
Internal server
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| Funding |
Yes
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| Dataset Access |
Limited
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| DUO Data Use Permission | |
| DUO Data Use Modifier | |
| DUO Investigation | |
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
2
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|---|---|
| Types Of Groups |
SOD1-G93A , WT
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| Overall Sample Size |
18
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| Main Disease Category |
Disease of anatomical entity
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| Specific Disease Model |
Neurological
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| Organ Or Tissue |
Skeletal muscles, brain
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| Sample Size For Each Group |
6 SOD1-G93A mice, 6 wt mice
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| Randomization |
No
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| Blinding |
No
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| Species |
Mice
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| Strain |
C57BL/6J (SOD1-G93A transgenic)
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| Immune Status |
Immunocompetent
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| Sex |
Male
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| Age |
6 weeks old
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| Weight |
20 g
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| Genotype |
Heterozygous
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| Genetic Manipulation |
Transgenic overexpression
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| Gene |
SOD1
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Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
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|---|---|
| Blood Sampling |
No
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| Surgical Procedures Including Sham Surgery |
No
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| Pathogen Infection Intervention And Control |
No
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| Analgesic Plan To Relieve Pain Suffering And Distress |
No
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| Anesthesia For Imaging |
Yes
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| Anesthesia Type |
Gas
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| Anesthesia Drugs |
Isovet
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| Anesthesia Dose |
Induction- 3–4% isoflurane in O₂ ; Maintenance- 1–2% in O₂
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| Euthanasia |
Yes
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| Method |
Anesthetic overdose
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| Histology |
Yes
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| Tissues Collected Post Euthanasia |
Triceps brachii skeletal muscle
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| Imaging |
Yes
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| Frequency Of Imaging |
One time
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| Timing Of Imaging |
1 week from disease onset
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
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| Contrast Agent Chemical Drug |
[¹⁸F]DPA-714
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| Contrast Agent Dose |
200–800 GBq/μmol
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| Injection Volume |
150 µL
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| Route Of Administration |
Intravenous
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| Cell Lines |
No
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| Cell Line |
No
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| Provenance |
No
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Image Acquisition
| Instrument Vendor |
Sedecal
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|---|---|
| Instrument Type |
SuperArgus
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| Instrument Specifics |
Fov 47 mm (axial) × 60 mm (transaxial)
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Image Data
| Image Type |
Processed image
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|---|---|
| Image Scale |
SUV
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| Format Compression |
DICOM
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| Dimensions |
2D
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| Image Processing Methods |
Image recontruction
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| Image Reconstruction Algorithm |
OSEM
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Analyzed Data
| Data Used For Analysis |
SUV
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|---|---|
| Updated Year |
2025
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