Dataset ID: 27
Dataset Details
Study Design
| Study Design Background |
This is a preclinical molecular imaging study using hybrid PET MRI and PET CT modalities to evaluate HER2 expression in various tumor models. The study integrates in vivo imaging with temporal biodistribution analysis to assess tracer specificity and tumor microenvironment effects in both HER2-positive and HER2-negative xenografts.
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|---|---|
| Study Description |
Preclinical evaluation of [52 Mn]Mn-DOTAGA(anhydride)-trastuzumab
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| Study Type |
Preclinical
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| Study Subtype |
In vitro, in vivo, ex vivo
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Publication
| Paper Linked |
Yes
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|---|---|
| Paper Title |
HER2 expression in different cell lines at different inoculation sites assessed by [52 Mn]Mn-DOTAGA(anhydride)-trastuzumab
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| Paper Authors |
Ngo Minh Toan, Vágner Adrienn, Nagy Gábor, Ország Gábor, Nagy Tamás, Szoboszlai Zoltán, Csikos Csaba, Váradi Balázs, Trencsényi György, Tircsó Gyula, Garai Ildikó
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| Affiliation |
Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen Department of Nuclear Medicine, Medical Imaging Clinic, Clinical Centre, University of Debrecen Scanomed Ltd., Debrecen, Hungary Department of Physical Chemistry, Institute of Chemistry, Faculty of Science and Technology, University of Debrecen
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| Paper Journal |
Pathology and Oncology Research
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| Paper Year |
2025
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| Paper DOI | |
| Open Access |
Yes
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Study Component
| Multi Modality Images |
Yes
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|---|---|
| Imaging Modality |
PET CT, PET MRI
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| Imaging Sub Modality |
MicroCT (standard energy-integrating detector, absorption-based); T1w
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| Radiation |
Yes
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| Imaging Coverage |
Whole body
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| Imaging Target |
Receptor expression
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Dataset Information
| Institution |
University of Debrecen
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|---|---|
| PI |
Ildikó Garai
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| PI-ORCID | |
| Co PI |
Ngo Minh Toan
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| Country Of Institution |
Hungary
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| Country Of Imaging Facility |
Hungary
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| Funding |
Yes
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| Funding Agency |
Ministry for Culture and Innovation from the source of the National Research
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| Grant Number |
EKÖP-24-4 University Research Scholarship Program
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| Dataset Access |
Public
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| License |
 |
| DUO Data Use Permission |
DUO-0000042
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| DUO Data Use Modifier |
DUO-0000012
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| DUO Investigation |
DUO-0000039
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| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
3
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|---|---|
| Types Of Groups |
Treated1, treated2, treated3
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| Overall Sample Size |
8
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| Animal Condition |
Disease model
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| Disease Model |
Cancer
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| Organ Or Tissue |
Mammary gland, epithelial tissue
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| Sample Size For Each Group |
Treated1 3, treated2 2, treated3 3
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| Randomization |
No
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| Blinding |
No
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| Statistical Methods |
ANOVA with post-hoc Tukey’s test
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| Species |
Mice
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| Strain |
CB17 SCID and C57BL 6
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| Immune Status |
Immunedefficient and immunecompetent
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| Sex |
Female
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| Age |
16–24 weeks old
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| Weight |
22.90 ± 4.28 g
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| Source Of Animals |
Animalab Ltd., Hungary
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Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
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|---|---|
| Blood Sampling |
Yes
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| Blood Timing |
Once
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| Surgical Procedures Including Sham Surgery |
No (only tumor injection)
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| Target Organ Tissue |
Mammary gland
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| Pathogen Infection Intervention And Control |
No
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| Analgesic Plan To Relieve Pain Suffering And Distress |
No
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| Anesthesia For Imaging |
Yes
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| Anesthesia Type |
Gas
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| Anesthesia Drugs |
Forane
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| Anesthesia Dose | |
| Monitoring Regime |
Body temperature, respiratoy rate, cardiac rate
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| Euthanasia |
Yes
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| Method |
Euthanized with 5 % Forane
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| Histology |
Yes
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| Tissues Collected Post Euthanasia |
Mammary gland tissue
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| Timing Of Collection |
At euthanasia
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| Tissue Description |
Tumor xenograft tissue
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| Perfusion Method |
No
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| Imaging |
Yes
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| Frequency Of Imaging |
One-day experiment
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| Timing Of Imaging |
14 days after the tumor cell inoculation
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
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| Contrast Agent Chemical Drug |
[52 Mn]Mn-DOTAGA(anhydride)-trastuzumab
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| Contrast Agent Dose |
3.50 ± 0.59 MBq
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| Injection Volume |
Depends on the radioactive concentration
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| Route Of Administration |
Intravenous
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| Cell Lines |
Yes
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| Cell Line |
MDA-MB-HER2+, MDA-MB-468, B16F10
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| Provenance |
American Type Culture Collection (ATCC)
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| Cell Culture Medium |
DMEM
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| Housing Room |
SPF
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| Food Intake Measured |
No
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Image Acquisition
| Instrument Vendor |
Mediso Ltd.
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|---|---|
| Instrument Type |
Nano Scan® PET MRI 1T, nano Scan® PET CT
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| Instrument Specifics |
3T, FOV: 32 mm × 32 mm
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| Image Acquisition Parameters |
0.5 mm slice thickness, 20 ms repetition time, 2.6 ms echo time, and 20° flip angle or CT with 180 projections and 55 k Vp X-ray source
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| Correction |
Decay correction, attenuation correction
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| Raw Data |
Yes
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Image Data
| Image Type |
Processed image
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|---|---|
| Image Scale |
SUV
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| Format Compression |
DICOM
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| Dimensions |
2D, 3D
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| Field Of View |
Bore size: 16 cm, Transaxial FOV: 12 cm, Axial FOV: 10 cm
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| Dimension Extents |
296, 296, 35
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| Size Description |
296*0,2694, 296*0,2694,35*1,347
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| PI xel Voxel Size Description |
1.12 mm×1.12 mm×13 mm
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| Image Processing Methods |
Reconstruction, smoothing
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| Image Reconstruction Algorithm |
OSEM-LOR Spatial resolution with Tera-Tomo (3D OSEM): 0.7 mm, Spatial Resolution with FBP (NEMA): 1.25 mm
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| Image Registration Algorithm |
Yes
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Analyzed Data
| Data Used For Analysis |
SUV
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|---|---|
| Updated Year |
2025
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