Dataset ID: 28
Dataset Details
Study Design
| Study Design Background |
1H MRS validation of the olfactory bulbectomised rats (model of major depressive disorder); perfusion measurement included
|
|---|---|
| Study Description |
Olfactory bulbectomy evaluated using 1H MRS and ASL methods
|
| Study Type |
Preclinical
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| Study Subtype |
In vivo
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Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Laterality in functional and metabolic state of the bulbectomised rat brain detected by ASL and 1H MRS: A pilot study
|
| Paper Authors |
Pavlova, I (Pavlova, Iveta) [1] , [2] ; Drazanova, E (Drazanova, Eva) [1] , [3] ; Kratka, L (Kratka, Lucie) [1] ; Amchova, P (Amchova, Petra) [3] ; Macicek, O (Macicek, Ondrej) [1] ; Starcukova, J (Starcukova, Jana) [1] ; Starcuk, Z (Starcuk, Zenon) [1] ; Ruda-Kucerova, J (Ruda-Kucerova, Jana) [3]
|
| Affiliation |
1 Czech Acad Sci, Inst Sci Instruments, Brno, Czech Republic, 2 Masaryk Univ, Fac Sci, Dept Condensed Matter Phys, Brno, Czech Republic, 3 Masaryk Univ, Fac Med, Dept Pharmacol, Kamenice 5, Brno 62500, Czech Republic
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| Paper Journal |
WORLD JOURNAL OF BIOLOGICAL PSYCHIATRY
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| Paper Year |
2023
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| Paper DOI | |
| Open Access |
No
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Study Component
| Multi Modality Images |
Yes
|
|---|---|
| Imaging Modality |
MRI
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| Imaging Sub Modality |
T1w, ASL, 1H MRS (PRESS)
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| Radiation |
No
|
| Imaging Coverage |
Brain
|
| Imaging Target |
Neurochemical profile - neuronal metabolism
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Dataset Information
| Institution |
Institute of Scientific Instruments of the Czech Academy of Sciences
|
|---|---|
| PI |
Iveta Harastova-Pavlova (ex Pavlova as named in the publication)
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| Country Of Institution |
Czech Republic
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| Imaging Facility |
Institute of Scientific Instruments Magnetic Resonance
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| Euro Bio Imaging Node |
Multimodel Imaging Brno Node
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| Country Of Imaging Facility |
Czech Republic
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| Link To Dataset |
Internal server
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| Funding |
Yes
|
| Funding Agency |
Ministry of Education, Youth & Sports - Czech Republic
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| Grant Number |
LM2015062 CZ.02.1.01/0.0/0.0/18_046/00016045
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| Dataset Access |
Limited
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| DUO Data Use Permission | |
| DUO Data Use Modifier | |
| DUO Investigation | |
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
2
|
|---|---|
| Types Of Groups |
CTR (controls), OBX (olfactory bulbectomised)
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| Overall Sample Size |
22
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| Animal Condition |
Both
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| Main Disease Category |
Disease of mental health
|
| Specific Disease Model |
Model of depression
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| Organ Or Tissue |
Brain
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| Sample Size For Each Group |
n CTR = 10, n OBX = 12
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| Randomization |
Yes; at the beginning of the study when splitting into the two groups
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| Blinding |
No
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| Species |
Rats
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| Strain |
Wistar
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| Immune Status |
Immunocompetent
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| Sex |
Male
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| Age |
Adult (~10 weeks)
|
| Age At Start Experiment |
77
|
| Age At Scanning Experiment S |
105-110
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| Weight |
340
|
| Genotype |
Unspecified
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| Genetic Manipulation |
No
|
Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
No
|
| Surgical Procedures Including Sham Surgery |
Yes
|
| Description Of The Surgical Procedure |
Bilateral ablation of the olfactory bulbs (under isoflurane anaesthesia): two 2mm diameter burr holes were drilled in the frontal bone, 7mm anterior and 2mm lateral from the bregma. Both olfactory bulbs were removed by aspiration. Blood loss prevention from the ablation cavity was achieved by filling the dead space with a haemostatic sponge. SHAM rats underwent identical anaesthetic and drilling procedures but with intact bulbs.
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| Target Organ Tissue |
Olfactory bulbs
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| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
Yes
|
| Analgesic Name |
Meloxicam
|
| Anesthesia For Imaging |
Yes
|
| Anesthesia Type |
Gas
|
| Anesthesia Drugs |
Isoflurane
|
| Anesthesia Dose |
2% isoflurane in 1000 mL/min of oxygen
|
| Monitoring Regime |
~50 br/min
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| Euthanasia |
Yes
|
| Method |
Anaesthetic overdose followed by head disection
|
| Histology |
No
|
| Imaging |
Yes
|
| Frequency Of Imaging |
Once
|
| Timing Of Imaging |
60 min (PRESS) + 20 min (ASL)
|
| Overall Scan Length |
120
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
No
|
| Cell Lines |
No
|
Image Acquisition
| Instrument Vendor |
Bruker
|
|---|---|
| Instrument Type |
Bruker BioSpec 94/30 USR
|
| Instrument Specifics |
9.4 T
|
| Correction |
Drift compensation, eddy current correction
|
| Raw Data |
Yes
|
| QA/QC |
Yes
|
Image Data
| Image Type |
T1w anatomical images, raw perfusion images, perfusion maps, primary spectra (FIDs)
|
|---|---|
| Image Scale |
Signal intensity, mL/min in 100 g of tissue, amplitude (in spectra)
|
| Format Compression |
2dseq
|
| Dimensions |
1D (spectra), 2D, 3D
|
| Overall Number Of Images |
264
|
| Field Of View |
3.7 x 3.7 (T1w, ASL), ~11 ppm (PRESS)
|
| Dimension Extents |
1.25 mm thick slices (in ASL and T1w ref. for ASL), 1.07 Hz/pt (specral res.)
|
| Size Description |
3.7 x 3.7 x 1.25 mm^3, voxel size in PRESS: 1.5 x 1.5 x 4 mm^3 (in hippocampus), 3 x 1 x 4 mm^3 (in sensorimotor cortex)
|
| PI xel Voxel Size Description |
128 x 64 x 1 (in ASL)
|
| Image Processing Methods |
No
|
| Quality Control |
No
|
| Ai Enhanced Algorithm |
No
|
Analyzed Data
| Data Used For Analysis |
Perfusion values in specific brain regions - home made software (code in matlab), relative metabolite concentrations
|
|---|---|
| Analysis Method And Details |
jMRUI
|
| Updated Year |
2026
|