Dataset ID: 3
Dataset Details
Study Design
| Study Design Background |
Study of the effectiveness of two different gamma-irradiated bone graft materials for bone regeneration in rat femurs. Ex vivo imaging was conducted at three time points after surgical induction of femur defect (drill hole defect, 3 mm diameter at mid-diaphysis), followed by defect filling with two types of composites. In vivo micro-CT imaging was also conducted after surgery and before each study time point (before euthanasia) with the goal of qualitatively monitoring the bone healing process on a macroscopic scale
|
|---|---|
| Study Description |
Tissue Lab
|
| Study Type |
Preclinical
|
| Study Subtype |
Ex-vivo
|
Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Evaluation of the Effect of a Gamma Irradiated DBM-Pluronic F127 Composite on Bone Regeneration in Wistar Rat
|
| Paper Authors |
Al Kayal, Tamer, Panetta, Daniele, Canciani, Barbara, Losi, Paola, Tripodi, Maria, Burchielli, Silvia, Ottoni, Priscilla, Salvadori, Piero Antonio, Soldani, Giorgio
|
| Affiliation |
Institute of Clinical Physiology- CNR, Pisa, Italy, University & IRCCS AOU San Martino—IST, National Institute for Cancer Research, DIMES, Genova, Italy, CNR Tuscany “G. Monasterio” Foundation, Pisa Italy
|
| Paper Journal |
Plos ONE
|
| Paper Year |
2015
|
| Paper DOI | |
| Open Access |
Yes
|
Study Component
| Multi Modality Images |
No
|
|---|---|
| Imaging Modality |
CT
|
| Radiation |
Yes
|
| Imaging Coverage |
Femur
|
| Imaging Target |
Bone healing
|
Dataset Information
| Institution |
CNR IFC, Pisa
|
|---|---|
| PI |
Daniele Panetta
|
| Country Of Institution |
Italy
|
| Imaging Facility |
CNR IFC, Pisa
|
| Euro Bio Imaging Node |
MMMI Italy
|
| Country Of Imaging Facility |
Italy
|
In Vivo Experimental Parameters
| Number Of Groups |
2
|
|---|---|
| Types Of Groups |
Graft materials: demineralized bone matrix (DBM) + Pluronic(TM) F127 (group 1), Pluronic F127 only (group 2)
|
| Overall Sample Size |
36
|
| Disease Model |
Bone disease
|
| Organ Or Tissue |
Femur
|
| Sample Size For Each Group |
18 rats per type of graft material. On each group, 3 subgroups of 6 animals each were used to test three different time points (15, 30 and 90 days from surgery)
|
| Randomization |
Yes
|
| Blinding |
No
|
| Outcome Measures |
Bone volume (BV) and bone surface to bone volume ratio (BS BV) inside three different volumes of interest (VOI) centered on the drill hole defects
|
| Statistical Methods |
T-test, 2-tails (group 1 vs group 2 at each time point)
|
| Species |
Rats
|
| Strain |
Wistar
|
| Sex |
Male
|
| Age |
3 months
|
| Age At Start Experiment |
3 months
|
| Weight |
Ca. 300 g
|
| Source Of Animals |
Harlan Laboratories S.r.l. (Udine, Italy)
|
| Registry Number Of Animal Authorization |
250 2011-B
|
Experimental Procedures
| Pharmacological Drug |
20% (w v) solution of Pluronic F127 in saline solution; DBM-PF127 composite, prepared mixing DBM particles (<80 um) and PF127 solution at 4°C to obtain a composite containing 40% (w w) DBM
|
|---|---|
| Company |
Tissue Lab srl (DBM); Sigma-Aldrich, MO, USA (PF127)
|
| Volume |
0,005
|
| Site Or Route Of Administration |
Local, on bone defect (one side only, the contralateral femur is kept empty for control)
|
| Frequency Of Administration |
Once, at the time of surgery
|
| Blood Sampling |
No
|
| Surgical Procedures Including Sham Surgery |
Yes
|
| Description Of The Surgical Procedure |
Drilling hole defect on each femur, mid-diaphysis, 3 mm diameter
|
| Target Organ Tissue |
Femur
|
| Pathogen Infection Intervention And Control |
No
|
| Anesthesia Type |
Intraperitoneal
|
| Duration |
3
|
| Anesthesia Drugs |
Zoletil+Xilazine
|
| Monitoring Regime |
Visual surveillance
|
| Euthanasia |
Yes
|
| Method |
Isoflurance inhalation
|
| Histology |
Yes
|
| Tissues Collected Post Euthanasia |
Femurs
|
| Timing Of Collection |
At each time point after euthanasia (15, 30 and 90 days post-surgery)
|
| Histological Procedure |
Fixed (formalin)
|
| Frequency Of Imaging |
In vivo (qualitative monitoring only): two times (post-surgery and at selected time point). Ex-vivo: single event
|
| Timing Of Imaging |
After fixation
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
No
|
Image Acquisition
| Instrument Vendor |
Custom made
|
|---|---|
| Instrument Type |
XALT-HR micro-CT
|
| Instrument Specifics |
Variable-magnification micro-CT operating in cone-beam geometry
|
| Image Acquisition Parameters |
50 kV, 0.7 mA, 1 mm Al filtration
|
| Correction |
Dark signal, flat-field equalization, ring artifact suppression
|
| Raw Data |
Yes
|
Image Data
| Image Type |
Primary image (CT)
|
|---|---|
| Image Scale |
Hounfield Units
|
| Format Compression |
Analyze 7.5
|
| Dimensions |
3D
|
| Overall Number Of Images |
1
|
| Field Of View |
18 mm x 30 mm (transaxial x axial)
|
| Dimension Extents |
700x700x1300
|
| Size Description |
13 mm x 13 mm x 24 mm
|
| PI xel Voxel Size Description |
18,4 um x 18,4 um x 18,4 um
|
| Image Reconstruction Algorithm |
FBP (ram-lak)
|
| Ai Enhanced Algorithm |
No
|
Analyzed Data
| Status |
Complete
|
|---|---|
| Updated Year |
2022
|