Dataset Details

Study Design

Study Design Background	Study of the causal role of the gut microbiota on cognitive development and cardiometabolic health. Germ-free mice were transplanted by oral gavage with fecal material obtained from 5 year old children, with gender coherence between donor and recipients. Mice body growth, food intake and cognitive function ware monitored for 5 months after fecal material transplantation (FMT). Then, total body positron emission tomography (microPET) coupled with FDG, and computed tomography (microCT) scans were run to measure glucose metabolism and radiodensity in several organs, with focus on brain, adipose tissues, myocardium, liver, gut. Ultrasound was also applied to measure cardiac morphological and functional parameters, and to assess hepatic lipid content.
Study Description	GUTMOM
Study Type	Preclinical
Study Subtype	In vivo and ex vivo

Publication

Paper Linked	In preparation

Study Component

Multi Modality Images	Yes
Imaging Modality	PET,CT,US NCIT:C17204 NCIT:C17230
Imaging Coverage	"PET CT total body; US heart and liver"
Imaging Target	Glucose metabolism (PET), anatomy (CT), lipid content (US, CT), cardiac function (US)

Dataset Information

In Vivo Experimental Parameters

Number Of Groups	4
Types Of Groups	Male transplanted, female transplanted, male sham, female sham
Overall Sample Size	72
Disease Model	Cardiac, neurological DOID:0014667
Organ Or Tissue	Brain, adipose tissue, liver, myocardium, gut NCIT:C12439 NCIT:C12472 NCIT:C12392 NCIT:C34082 NCIT:C12371
Sample Size For Each Group	30 males transplanted, 30 females transplanted, 6 males sham, 6 females sham
Randomization	Yes, mice were randomly assigned to groups
Blinding	No
Outcome Measures	FDG fractional extraction and glucose metabolism of brain, adipose tissue, liver, myocardium, gut; tissues radiodensity, adipose tissue volumes, hepatic lipid content, cardiac function
Statistical Methods	One-way ANOVA, 2-tails t-test
Species	Mice NCIT:C14238
Strain	C57BL 6 NCIT:C14424
Sex	Males and females (50 50%)
Age	4-5 weeks
Age At Start Experiment	4-5 weeks
Age At Scanning Experiment S	About 6 months
Weight At Start Experiment	About 20 g
Weight At End Experiment	About 30 g
Source Of Animals	Charles River Laboratories s.r.l. (Milano, Italy)
Registry Number Of Animal Authorization	464 2018-PR

Experimental Procedures

Pharmacological Drug	Fecal material, prepared in PBS (100 mg of stool in 0.77 ml if sterile PBS, vortex and filterd). The filtrated was adminsterd
Volume	0,15
Site Or Route Of Administration	Oral gavage
Frequency Of Administration	Once, at the beginnig of the protocol
Vehicle Or Carrier Solution Formulation	PBS
Blood Sampling	Yes
Blood Sample Volume	About 300 ml
Blood Timing	Once, after the animal was sacrificed
Surgical Procedures Including Sham Surgery	No
Anesthesia Type	Gas
Duration	100
Anesthesia Drugs	Isoflurane
Anesthesia Dose	1-2%
Monitoring Regime	Visual surveillance and cardiac rate
Euthanasia	Yes
Method	Isoflurane overdose
Histology	Yes
Tissues Collected Post Euthanasia	All organs
Timing Of Collection	Immediately after PET-CT imaging
Histological Procedure	Fixed (formalin)
Frequency Of Imaging	Single event
Timing Of Imaging	5 months after fecal material transplantation, at the end of the in vivo protocol
Overall Scan Length	100
Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule	Yes
Contrast Agent Chemical Drug	18F-fluorodeoxyglucose (FDG) CHEBI:49134
Contrast Agent Dose	6.6 MBq
Injection Volume	0,26
Route Of Administration	Intraperitoneal

Analyzed Data

Status	Ongoing
Updated Year	2022