Dataset ID: 7
Dataset Details
Study Design
| Study Design Background |
Inflammation assessment by in vivo PET and MRI of a Parkinson's disease model (MPTP) using the TSPO specific radiotracer [18F]VC701. Implementation of new tool analysis for mouse brain PET fully based on CT-images
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|---|---|
| Study Description |
Molecular and imaging prodromal markers of neurodegeneration in Parkinson disease
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| Study Type |
Preclinical
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| Study Subtype |
In vivo
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Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
Development of a new toolbox for mouse PET-CT brain image analysis fully based on CT images and validated in a PD mouse model
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| Paper Authors |
Presotto L, Bettinardi V, Mercatelli D, Picchio M, Morari M, Moresco RM and Belloli S
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| Affiliation |
IBFM-CNR, University of Milano - Bicocca, IRCCS San Raffaele of Milan, University of Ferrara, Italy
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| Paper Journal |
Scientific reports
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| Paper Year |
2022
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| Paper DOI | |
| Open Access |
Yes
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Study Component
| Multi Modality Images |
Yes
|
|---|---|
| Imaging Modality |
PET CT,MRI
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| Imaging Sub Modality |
T2w MRI
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| Radiation |
Yes
|
| Imaging Coverage |
CT- head; PET - whole body
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| Imaging Target |
TSPO receptor activity
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Dataset Information
| Institution |
IBFM-CNR
|
|---|---|
| PI |
Moresco Rosa Maria
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| Co PI |
Belloli Sara
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| Country Of Institution |
Italy
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| Imaging Facility |
Laboratory of molecular imaging for small animal
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| Euro Bio Imaging Node |
MMMI Italy
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| Country Of Imaging Facility |
Italy
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In Vivo Experimental Parameters
| Number Of Groups |
1
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|---|---|
| Types Of Groups |
Pre- e post-MPTP (1-metil 4-fenil 1,2,3,6-tetraidro-piridina) in a test-retest study
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| Overall Sample Size |
6
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| Disease Model |
Parkinson's disease
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| Organ Or Tissue |
Brain
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| Sample Size For Each Group |
6 mice pre and post-treated with MPTP
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| Power Calculation |
None, pilot study
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| Randomization |
Single group
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| Blinding |
No
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| Statistical Methods |
Student's t test for paired samples
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| Species |
Mice
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| Strain |
C57BL 6
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| Immune Status |
Immunocompetent
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| Sex |
Male
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| Age |
Three-month-old
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| Age At Start Experiment |
Three-month-old
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| Age At Scanning Experiment S |
Three-month-old
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| Weight |
About 30 g
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| Weight At Start Experiment |
About 30 g
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| Weight At End Experiment |
About 30 g
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| Source Of Animals |
University of Ferrara, Italy
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| Registry Number Of Animal Authorization |
237 2019-PR
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Experimental Procedures
| Pharmacological Drug |
MPTP
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|---|---|
| Company |
Med Chem Express
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| Formulation |
Solution from powder
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| Drug Dose |
30
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| Volume |
0,3
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| Concentration |
100 mg ml
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| Site Or Route Of Administration |
Intraperitoneally
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| Frequency Of Administration |
Daily for 7 days
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| Vehicle Or Carrier Solution Formulation |
Saline
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| Drug Or Batch Sample Number |
Lot # 27361
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| Blood Sampling |
No
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| Surgical Procedures Including Sham Surgery |
No
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| Pathogen Infection Intervention And Control |
No
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| Analgesic Plan To Relieve Pain Suffering And Distress |
No
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| Anesthesia Type |
Gas
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| Duration |
30
|
| Anesthesia Drugs |
Isofluran
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| Anesthesia Dose |
2% in 1L per minute air mix
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| Monitoring Regime |
Software remote control of the animal's vital functions (temperature, heartbeat and breathing)
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| Euthanasia |
Yes
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| Method |
CO2 plus cervical dislocation
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| Histology |
No
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| Frequency Of Imaging |
Two time points
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| Timing Of Imaging |
Pre and post-MPTP treatment
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| Overall Scan Length |
25 min for PET-CT and 70 min for MRI
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| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
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| Contrast Agent Commercial Drug |
[18F]VC701
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| Contrast Agent Chemical Drug |
Fluoromethyl derivative of PK11195
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| Contrast Agent Dose |
Mean injected activity: 4,7 ± 0,3 MBq
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| Injection Volume |
0,03
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| Injection Time |
Scanning 100 min after radiotracer's injection
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| Vehicle |
Saline
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| Route Of Administration |
Intravenous
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Image Acquisition
| Instrument Vendor |
PET-CT Molecubes; MRI Bruker
|
|---|---|
| Instrument Type |
Î’-Cube and X-Cube for PET and CT, respectively;Bruker Biospec 70 30 for MRI
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| Instrument Specifics |
PET- FOV of 72x130 mm; CT: FOV of 64x40 mm; 7 Tesla MRI equipped with 450 675 mT m gradients
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| Image Acquisition Parameters |
PET- 511 KeV, 30% energy resolution; CT: 40 k Vp, 400 ?A (0.8 mm Al filtration)
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| Correction |
PET- isotope decay and random coincidences correction; CT: ring artifact correction
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| Raw Data |
Yes
|
| QA/QC |
Yes. PET- daily and mothly QC; CT- monthly QC; MRI- daily QC
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Image Data
| Image Type |
Reconstructed and quantified images
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|---|---|
| Image Scale |
KBq cc for PET and HU for CT, T2 relaxation time for MRI
|
| Format Compression |
Raw, DICOM and NIFTI
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| Dimensions |
2D 3D
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| Overall Number Of Images |
1 dataset per animal per imaging technique per time point
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| Field Of View |
Max FOV for PET and CT, 14x15 mm for MRI
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| Dimension Extents |
PET- 192x192x384; MRI: 256x96x102
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| Size Description |
PET- 76.8 x 76.8 x 154 mm; MRI: 18,7 x 7 x 15 mm
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| PI xel Voxel Size Description |
PET- 400 µm isometric; MRI: 73 x 73 x 600 µm
|
| Image Processing Methods |
CT-based normalization
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| Image Reconstruction Algorithm |
PET- OSEM (30 iterations); CT: ISRA (iterative).
|
| Quality Control |
No
|
| Image Registration Algorithm |
No
|
| Ai Enhanced Algorithm |
No
|
| Corrections |
PET- acquisition decay, randoms; CT: none
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Image Correlation
| Spatial And Temporal Alignment |
PET and CT images were automatically co-registered by the software; MR images were co-registered using CT
|
|---|---|
| Fiducials Used |
Brain volume
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| Coregistered Images |
Yes
|
| Transformation Matrix Other Info |
SPM coregistration
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Analyzed Data
| Analysis Result Type |
Regional increase of radiotracer's uptake
|
|---|---|
| Data Used For Analysis |
VOIs analysis, p value
|
| Analysis Method And Details |
SPM, PMOD
|
| Status |
Complete
|
| Updated Year |
2022
|