Dataset ID: 9
Dataset Details
Study Design
| Study Design Background | Evaluation of new MRI-CEST contrast agent based on glucose |
|---|---|
| Study Description | GLINT glucose-3OMG comparison |
| Study Type | Preclinical |
| Study Subtype | In vivo |
Publication
| Paper Linked | Yes |
|---|---|
| Paper Title | In vitro and in vivo comparison of MRI chemical exchange saturation transfer (CEST) properties between native glucose and 3-O-Methyl-D-glucose in a murine tumor model |
| Paper Authors | Anemone A, Capozza M, Arena F, Zullino S, Bardini P, Terreno E, Longo DL, Aime S. |
| Affiliation | Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy. |
| Paper Journal | NMR Biomed |
| Paper Year | 2021 |
| Paper DOI | https://doi.org/10.1002/nbm.4602 |
| Open Access | Yes |
Study Component
| Multi Modality Images | No |
|---|---|
| Imaging Modality | MRI |
| NCIT Imaging | |
| Imaging Sub Modality | CEST |
| NCIT Imaging Submodality | |
| Radiation | No |
| Imaging Coverage | Body |
| Imaging Target | Glucose transporter (GLUT) |
Dataset Information
| Institution | IBB-CNR |
|---|---|
| PI | Dario Longo |
| Country Of Institution | Italy |
| Euro Bio Imaging Node | MMMI |
| Country Of Imaging Facility | Italy |
| Link To Dataset | https://eubi-xnat.hpc4ai.unito.it/app/template/XDATScreen_report_xnat_projectData.vm/search_element/xnat:projectData/search_field/xnat:projectData.ID/search_value/glint_glucose-3OMG |
| Contact Person | dario.longo@unito.it |
In Vivo Experimental Parameters
| Number Of Groups | 4 |
|---|---|
| Types Of Groups | Glucose injected low dose, glucose injected high dose, 3OMG injected low dose, 3OMG injected high dose |
| Overall Sample Size | 20 |
| Disease Model | Cancer |
| DOID | |
| Organ Or Tissue | Melanoma |
| Sample Size For Each Group | 5, 5, 5, 5 |
| Randomization | No |
| Blinding | No |
| Outcome Measures | CEST contrast, tumor volume |
| Statistical Methods | Unpaired Student's t-test |
| Species | Mice |
| NCIT Species | |
| Strain | C57 BL6 |
| NCIT Strain | |
| Immune Status | Immunocompetent |
| Sex | Male |
| Age | 8 weeks |
| Age At Start Experiment | 8 weeks |
| Weight | 25 g |
| Source Of Animals | Charles River Laboratories, Calco, Italy |
Experimental Procedures
| Pharmacological Procedures Intervention And Control | No |
|---|---|
| Blood Sampling | No |
| Surgical Procedures Including Sham Surgery | No |
| Pathogen Infection Intervention And Control | No |
| Analgesic Plan To Relieve Pain Suffering And Distress | No |
| CHEBI Anesthesia | |
| Anesthesia Type | Gas |
| Duration | 45 |
| Anesthesia Drugs | Isofluran |
| Anesthesia Dose | 1-2% |
| Monitoring Regime | Respiratoy rate |
| Euthanasia | Yes |
| Method | Cervical dislocation |
| Histology | No |
| Frequency Of Imaging | Single scan |
| Overall Scan Length | 45 |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | Yes |
| Contrast Agent Commercial Drug | Glucose, 3OMG |
| CHEBI Contrast Agent Commercial Name | |
| Contrast Agent Chemical Drug | Glucose, 3OMG |
| CHEBI Contrast Agent Chemical Name | |
| Contrast Agent Dose | 1.5 g kg, 3.0 g kg |
| Injection Volume | 0,2 |
| Injection Time | 20 seconds |
| Vehicle | Saline |
| Route Of Administration | Intravenous |
| Cell Line | B16-F10 |
| CLO | |
| Provenance | Collaborator |
| Cell Injection Route | Subcutaneous |
| Number Of Cells | 500000 |
| Housing Room | Not SPF |
| Dietary Intervention | No |
| Food Intake Measured | No |
Image Acquisition
| Instrument Vendor | Bruker |
|---|---|
| Instrument Type | Pharmascan 70 16, Biospec |
| Instrument Specifics | 7T, 3T |
| Correction | No |
| Raw Data | Yes |
| QA/QC | No |
Image Data
| Image Type | Primary image processed images |
|---|---|
| Format Compression | Raw; DICOM |
| Dimensions | 2D + time |
| Field Of View | 30x30x2 mm |
| Dimension Extents | 64x64x1 |
| Size Description | 30x30x2 mm |
| PI xel Voxel Size Description | 0.468x0.468x2 mm |
| Image Processing Methods | Default reconstruction |
| Quality Control | No |
| Image Registration Algorithm | No |
| Ai Enhanced Algorithm | Yes |
| QC Info | R2 calculation on raw data, CEST contrast |
Analyzed Data
| Analysis Result Type | Numerical |
|---|---|
| Data Used For Analysis | CEST contrast |
| Analysis Method And Details | Manual delineation of tumor regions |
| Status | Complete |
| Updated Year | 2022 |