Dataset ID: 9
Dataset Details
Study Design
| Study Design Background |
Evaluation of new MRI-CEST contrast agent based on glucose
|
|---|---|
| Study Description |
GLINT glucose-3OMG comparison
|
| Study Type |
Preclinical
|
| Study Subtype |
In vivo
|
Publication
| Paper Linked |
Yes
|
|---|---|
| Paper Title |
In vitro and in vivo comparison of MRI chemical exchange saturation transfer (CEST) properties between native glucose and 3-O-Methyl-D-glucose in a murine tumor model
|
| Paper Authors |
Anemone A, Capozza M, Arena F, Zullino S, Bardini P, Terreno E, Longo DL, Aime S.
|
| Affiliation |
Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy.
|
| Paper Journal |
NMR Biomed
|
| Paper Year |
2021
|
| Paper DOI | |
| Open Access |
Yes
|
Study Component
| Multi Modality Images |
No
|
|---|---|
| Imaging Modality |
MRI
|
| Imaging Sub Modality |
CEST
|
| Radiation |
No
|
| Imaging Coverage |
Body
|
| Imaging Target |
Glucose transporter (GLUT)
|
Dataset Information
| Institution |
IBB-CNR
|
|---|---|
| PI |
Dario Longo
|
| Country Of Institution |
Italy
|
| Euro Bio Imaging Node |
MMMI
|
| Country Of Imaging Facility |
Italy
|
| Link To Dataset | |
| Contact Person |
In Vivo Experimental Parameters
| Number Of Groups |
4
|
|---|---|
| Types Of Groups |
Glucose injected low dose, glucose injected high dose, 3OMG injected low dose, 3OMG injected high dose
|
| Overall Sample Size |
20
|
| Disease Model |
Cancer
|
| Organ Or Tissue |
Melanoma
|
| Sample Size For Each Group |
5, 5, 5, 5
|
| Randomization |
No
|
| Blinding |
No
|
| Outcome Measures |
CEST contrast, tumor volume
|
| Statistical Methods |
Unpaired Student's t-test
|
| Species |
Mice
|
| Strain |
C57 BL6
|
| Immune Status |
Immunocompetent
|
| Sex |
Male
|
| Age |
8 weeks
|
| Age At Start Experiment |
8 weeks
|
| Weight |
25 g
|
| Source Of Animals |
Charles River Laboratories, Calco, Italy
|
Experimental Procedures
| Pharmacological Procedures Intervention And Control |
No
|
|---|---|
| Blood Sampling |
No
|
| Surgical Procedures Including Sham Surgery |
No
|
| Pathogen Infection Intervention And Control |
No
|
| Analgesic Plan To Relieve Pain Suffering And Distress |
No
|
| Anesthesia Type |
Gas
|
| Duration |
45
|
| Anesthesia Drugs |
Isofluran
|
| Anesthesia Dose |
1-2%
|
| Monitoring Regime |
Respiratoy rate
|
| Euthanasia |
Yes
|
| Method |
Cervical dislocation
|
| Histology |
No
|
| Frequency Of Imaging |
Single scan
|
| Overall Scan Length |
45
|
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule |
Yes
|
| Contrast Agent Commercial Drug |
Glucose, 3OMG
|
| Contrast Agent Chemical Drug |
Glucose, 3OMG
|
| Contrast Agent Dose |
1.5 g kg, 3.0 g kg
|
| Injection Volume |
0,2
|
| Injection Time |
20 seconds
|
| Vehicle |
Saline
|
| Route Of Administration |
Intravenous
|
| Cell Line |
B16-F10
|
| Provenance |
Collaborator
|
| Cell Injection Route |
Subcutaneous
|
| Number Of Cells |
500000
|
| Housing Room |
Not SPF
|
| Dietary Intervention |
No
|
| Food Intake Measured |
No
|
Image Acquisition
| Instrument Vendor |
Bruker
|
|---|---|
| Instrument Type |
Pharmascan 70 16, Biospec
|
| Instrument Specifics |
7T, 3T
|
| Correction |
No
|
| Raw Data |
Yes
|
| QA/QC |
No
|
Image Data
| Image Type |
Primary image processed images
|
|---|---|
| Format Compression |
Raw; DICOM
|
| Dimensions |
2D + time
|
| Field Of View |
30x30x2 mm
|
| Dimension Extents |
64x64x1
|
| Size Description |
30x30x2 mm
|
| PI xel Voxel Size Description |
0.468x0.468x2 mm
|
| Image Processing Methods |
Default reconstruction
|
| Quality Control |
No
|
| Image Registration Algorithm |
No
|
| Ai Enhanced Algorithm |
Yes
|
| QC Info |
R2 calculation on raw data, CEST contrast
|
Analyzed Data
| Analysis Result Type |
Numerical
|
|---|---|
| Data Used For Analysis |
CEST contrast
|
| Analysis Method And Details |
Manual delineation of tumor regions
|
| Status |
Complete
|
| Updated Year |
2022
|