Metadata
Dataset ID:
11
Study Design
| Study Design Background | To synthesis and evaluation of a PSMA-617-targeted dual NIRF/PDTprobe conceived using IRDye700DX as photosensitizer and FGS NIR dye. |
|---|---|
| Study Description | probe conceived using IRDye700DX as photosensitizer and FGS NIR dye |
| Study Type | preclinical |
| Study Subtype | in vivo/ex vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | A Novel PSMA-Targeted Probe for NIRF-Guided Surgery andPhotodynamic Therapy: Synthesis and Preclinical Validation |
| Paper Authors | Capozza, Martina; Stefania, Rachele; Dinatale, Valentina; Bitonto, Valeria; Conti, Laura; Grange, Cristina; Skovronova, Renata; Terreno, Enzo |
| Affiliation | Molecular & Preclinical Imaging Centers, Department of Molecular Biotechnology and Health Sciences, University of Torino, 10126 Torino, Italy ; Molecular Biotechnology Center, Department of Medical Science, University of Torino, 10126 Torino, Italy; Molecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, 10126 Torino, Italy |
| Paper Journal | IJMS |
| Paper Year | 2022 |
| Paper Doi | https://doi.org/10.3390/ijms232112878 |
| Open Access | yes |
| Updated Year | 2023 |
Study Component
| Multi Modality Images | yes |
|---|---|
| Imaging Modality | MRI-OI |
| Imaging Sub Modality | T2w and fluorescent imaging (filter pair: 640/720 nm) |
| Radiation | no |
| Imaging Coverage | body |
| Imaging Target | PSMA |
Dataset Information
| Institution | UniTO |
|---|---|
| Pi | Enzo Terreno |
| Country Of Institution | Italy |
| Imaging Facility | Laboratory of molecular imaging for small animal |
| Euro Bio Imaging Node | MMMI |
| Country Of Imaging Facility | Italy |
| Contact Person | martina.capozza@unito.it |
In Vivo Experimental Parameters
| Number Of Groups | 5 |
|---|---|
| Types Of Groups | 1nmol PSMA+ inj biod; 5nmol PSMA+ inj biod; 5 nmol PSMA- biod; PSMA+ PDT treated; PSMA+ Ctrl |
| Overall Sample Size | 25 |
| Disease Model | cancer |
| Organ Or Tissue | prostate |
| Sample Size For Each Group | 5, 5, 5, 5, 5 |
| Power Calculation | none |
| Randomization | mice were randomly divided into treted and control group |
| Blinding | no |
| Outcome Measures | fluorescent uptake, tumor volume |
| Statistical Methods | anova and t-test |
| Species | Mice |
| Strain | athymic nude |
| Immune Status | immunodeficient |
| Sex | male |
| Age | 5 weeks |
| Age At Start Experiment | 6 weeks |
| Age At Scanning Experiment S | older than 8 weeks |
| Weight | 20g |
| Weight At Start Experiment | 20 |
| Weight At End Experiment | 20 |
| Source Of Animals | envigo |
| Registry Number Of Animal Authorization | 229/2016 |
Experimental Procedures
| Pharmacological Drug | IRDye700DX-PSMA |
|---|---|
| Company | unito |
| Formulation | PBS |
| Drug Dose | 1-5 nmol/mice |
| Site Route Of Administration | iv |
| Frequency Of Administration | once |
| Vehicle Or Carrier Solution Formulation | PBS |
| Drug Batch Sample Number | na |
| Blood Sampling | no |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | intramuscolar |
| Duration | 45 |
| Anesthesia Drugs | Zoletil/Rompun |
| Anesthesia Dose | 20 mg/kg tiletamine/zolazepam (Zoletil 100; Virbac, Milan, Italy) and 5 mg/kg xylazine (Rompun; Bayer, Milan, Italy). |
| Euthanasia | yes |
| Method | cervival dislocation |
| Histology | yes |
| Tissues Collected Post Euthanasia | tumor |
| Timing Of Collection | different time points |
| Histological Procedure | optical cutting temperature (OCT) compound |
| Name Of Reagent S | anti-CD68, anti PSMA, anti CD31, |
| Frequency Of Imaging | 2 times/week MRI |
| Timing Of Imaging | three weeks after inoculation |
| Overall Scan Length | 20 min |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | no |
| Cell Line | PC3-PIP and PC3-FLU |
| Provenance | THE JOHNS HOPKINS UNIVERSITY |
| Modified Cell Line | yes |
| Type Of Genetic Modification | plasmid |
| Gene Modified | PSMA |
| Cell Injection Route | subcutaneous |
| Number Of Cells | 2x10^6 |
| Housing Room | SPF and not SPF |
| Dietary Intervention | diet for OI |
Image Acquisition
| Instrument Vendor | Bruker for MRI and Perkin Elmer for OI |
|---|---|
| Instrument Type | 7 T Bruker Pharmascan, IVIS spectrum |
| Instrument Specifics | 7T |
| Raw Data | yes |
| Qa Qc | no |
Image Correlation
| Image Scale | average radiance efficiency for OI |
|---|---|
| Format Compression | raw |
| Dimensions | 2D |
| Overall Number Of Images | 32 x MRI; 7 x OI |
| Field Of View | 30x30 for MRI; D for Ivis |
| Dimension Extents | 128x128 |
| Size Description | 30x30x2 mm |
| Image Processing Methods | default |
Analyzed Data
| Analysis Result Type | numerical |
|---|---|
| Data Used For Analysis | ROI |
| Analysis Method And Details | manual |
| File Format Of Result File Csv Json Txt Xlsx | xlsx |
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C16809 |
|---|---|
| Ncit Imaging Submodality | NCIT:C180729,NCIT:C116489 |
| Doid | DOID:162 |
| Ncit Anatomy | NCIT:C12410 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C14239 |
| Chebi Pharmaco | CHEBI:53287 |
| Chebi Anesthesia | CHEBI:92386 |
| Clo | CLO:0008395 |