Metadata
Dataset ID:
12
Study Design
| Study Design Background | To visualize LPS-induced neuroinflammation using paramagnetic micelles decorated with a peptide targeting the inflammation marker VCAM-1 |
|---|---|
| Study Description | VCAM-1 paramagnetic micelles to detect neuroinflammation by MRI |
| Study Type | preclinical |
| Study Subtype | in vivo/ex vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | MRI visualization of neuroinflammation using VCAM-1 targeted paramagnetic micelles. |
| Paper Authors | Garello Francesca, Pagoto Amerigo, Arena Francesca, Buffo Annalisa, Blasi Francesco, Alberti Diego, Terreno Enzo. |
| Affiliation | Molecular & Preclinical Imaging Centers, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Department of Neuroscience Rita Levi-Montalcini, University of Torino, Torino, Italy; Neuroscience Institute Cavalieri Ottolenghi, Torino, Italy; Molecular & Preclinical Imaging Centers, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy. |
| Paper Journal | Nanomedicine: Nanotechnology, Biology and Medicine |
| Paper Year | 2018 |
| Paper Doi | https://doi.org/10.1016/j.nano.2017.10.002. |
| Open Access | no |
| Updated Year | 2023 |
Study Component
| Multi Modality Images | no |
|---|---|
| Imaging Modality | MRI |
| Imaging Sub Modality | T1w CE, T2w |
| Radiation | no |
| Imaging Coverage | brain |
| Imaging Target | VCAM-1 |
Dataset Information
| Institution | UniTO |
|---|---|
| Pi | Enzo Terreno |
| Country Of Institution | Italy |
| Imaging Facility | Laboratory of molecular imaging for small animal |
| Euro Bio Imaging Node | MMMI |
| Country Of Imaging Facility | Italy |
| Contact Person | francesca.garello@unito.it |
In Vivo Experimental Parameters
| Number Of Groups | 3 |
|---|---|
| Types Of Groups | blood half-life determination, VCAM-1 micelles, scrambled micelles, Multihance |
| Overall Sample Size | 19 |
| Disease Model | neuroinflammation |
| Organ Or Tissue | brain |
| Sample Size For Each Group | 3 for half-life time, 8 for VCAM-1 micelles, 4 for scrambled micelles, 4 for Multihance |
| Power Calculation | none |
| Inclusion Criteria | none |
| Exclusion Criteria | none |
| Randomization | mice were randomly divided into treted and control group |
| Blinding | no |
| Outcome Measures | MRI T1 contrast |
| Statistical Methods | t-test |
| Species | Mice |
| Strain | C57/BL6 |
| Immune Status | Immunocompetent |
| Sex | female |
| Age | older than 8 weeks |
| Age At Start Experiment | 8 weeks |
| Age At Scanning Experiment S | 8 weeks |
| Weight | About 18-20 g |
| Weight At Start Experiment | About 18-20 g |
| Weight At End Experiment | About 18-20 g |
| Source Of Animals | University of Torino, Italy |
Experimental Procedures
| Pharmacological Procedures Intervention And Control | no |
|---|---|
| Blood Sampling | yes |
| Blood Sampling Method | from the tail vein |
| Blood Sample Volume | 20-50 ul |
| Blood Timing | 5, 15, 30 min, 1, 2, 4 and 24 h |
| Surgical Procedures Including Sham Surgery | yes |
| Description Of The Surgical Procedure | Acute neuroinflammatory response was induced in the right striatum. To obtain the model, mice were anesthetized, positioned in a stereotaxic apparatus (Stoelting) and the skull was exposed. The brain of the animal was then exposed via a hole, which was drilled through the skull. A glass capillary was inserted into the right striatum using the following stereotaxic coordinates from bregma: + 0.1 mm antero-posterior, ? 2.1 mm mediolateral, ? 2.6 mm dorsoventral. 1 ?L of lipopolysaccharide (LPS) (E. coli 026:B6, Sigma?Aldrich) in sterile phosphate buffered saline PBS at a concentration of 5 ?g/?l was then infused with a pneumatic pressure injection apparatus (Picospritzer II, General Valve Corporation) over 5 min followed by a 2 minutes rest period to allow the solution to diffuse into the brain prior to the removal of the capillary. For the saline control mouse, the same surgery described above was performed, but the vehicle only (1 ?L of PBS) was injected intra-striatally. |
| Target Organ Tissue | brain |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | gas |
| Duration | ~30 minutes |
| Anesthesia Drugs | Isoflurane |
| Anesthesia Dose | 1-2% isoflurane |
| Monitoring Regime | respiratoy rate |
| Euthanasia | yes |
| Method | cervival dislocation |
| Histology | yes |
| Tissues Collected Post Euthanasia | brain |
| Timing Of Collection | 24 h post VCAM-1 or scarmbled micelles injection |
| Histological Procedure | frozen |
| Name Of Reagent S | VCAM-1, F4/80, CD31 |
| Catalogue Number | rat anti-mouse CD31, Clone MEC 13:3, BD Pharmingen. Rat anti mouse F4/80 antibody, clone Cl:A3-1, Serotec. |
| Length Of Fixation | 5 min, absolute ethanol |
| Frequency Of Imaging | Pre, 20 min, 4 h, 24 h and 48 h post injection of micelles. |
| Timing Of Imaging | 24h post LPS administration. |
| Overall Scan Length | 20 min |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | contrast agent |
| Contrast Agent Commercial Drug | Multihance / paramagnetic micelles |
| Contrast Agent Chemical Drug | gadobenate dimeglumine |
| Contrast Agent Dose | VCAM-1 targeted or scrambled micelles (0.05 mmolGd/kg); MultiHance (0.2 mmolGd/Kg) |
| Injection Volume | 0,2 |
| Injection Time | 10 seconds |
| Vehicle | saline |
| Route Of Administration | intravenous |
| Housing Room | not SPF |
| Dietary Intervention | no |
| Respiration Rate | only during scanning |
| Body Tempurature Etc | only during scanning |
| Food Intake Measured | no |
Image Acquisition
| Instrument Vendor | Bruker |
|---|---|
| Instrument Type | Icon, equipped with a mouse brain volume coil |
| Instrument Specifics | 1T |
| Image Acquisition Parameters | T2-weighted MRI images were obtained using a RARE sequence protocol (TR/TE/NAV = 2500/26.7/12). T1-weighted MRI images were acquired using a FLASH sequence (TR/TE/NAV = 75/5.8/18, field of view (FOV) = 1.2 cm) |
| Raw Data | yes |
Image Correlation
| Image Type | Primary image |
|---|---|
| Dimensions | 2D |
| Field Of View | 1,2 cm x 1,2 cm |
| Dimension Extents | 128x128 |
| Image Processing Methods | default |
| Quality Control | no |
| Image Smoothing Or Filtering Algorithm | no |
| Image Registration Algorithm | no |
Analyzed Data
| Analysis Result Type | numerical |
|---|---|
| Data Used For Analysis | ROI |
| Analysis Method And Details | manual |
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C16809 |
|---|---|
| Ncit Imaging Submodality | NCIT:C180729,NCIT:C180727,NCIT:C46087 |
| Ncit Anatomy | NCIT:C12439 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C14424 |
| Chebi Anesthesia | CHEBI:6015 |