Metadata
Dataset ID:
13
Study Design
| Study Design Background | This study investigated the potential of 1H relaxometry and 23Na MRI to monitor water fluxes and dysregulation of Na+ homeostasis as novel target for therapeutic intervention. The effect of a forced Na+ influx induced by the ionophore monensin on hepatocellular carcinoma models was compared with that on hepatocytes and normal tissues |
|---|---|
| Study Description | The effect of monensin treatment in vitro and in vivo in hepatocellular carcinoma models |
| Study Type | preclinical |
| Study Subtype | in vivo/ex vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | Boosting intracellular sodium selectively kills hepatocarcinoma cells and induces hepatocellular carcinoma tumor shrinkage in mice. |
| Paper Authors | Nausicaa Clemente1, Simona Baroni2, Simone Fiorilla1, Francesco Tasso1, Simone Reano3, Chiara Borsotti1, Maria Rosaria Ruggiero2, Elisa Alchera4, Marco Corrazzari5, Gillian Walker1, Antonia Follenzi1, Simonetta Geninatti Crich2 and Rita Carini1 |
| Affiliation | Department of Health Science University del Piemonte Orientale, Novara, Italy; Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Department of Translational Medicine, Unit of Muscle Biology, Università del Piemonte Orientale, Novara, Italy; Division of Experimental Oncology/Unit of Urology, URI, IRCCS, Ospedale San Raffaele, Milan, Italy; Department of Health Science and Interdisciplinary Research Center of Autoimmune Disease (IRCAD), Università del Piemonte Orientale, Novara, Italy. |
| Paper Journal | Communications Biology |
| Paper Year | 2023 |
| Paper Doi | under pubblication |
| Open Access | yes |
| Updated Year | 2023 |
Study Component
| Multi Modality Images | no |
|---|---|
| Imaging Modality | MRI |
| Imaging Sub Modality | Surface coil 1H T2w, 23Na Gradient-echo FLASH |
| Radiation | no |
| Imaging Coverage | sub-cutaneous tumor in the back |
| Imaging Target | Anatomy, Sodium tissue content |
Dataset Information
| Institution | UniTO |
|---|---|
| Pi | S. Genibatti Crich |
| Country Of Institution | Italy |
| Imaging Facility | Laboratory of molecular imaging for small animal |
| Euro Bio Imaging Node | MMMI |
| Country Of Imaging Facility | Italy |
| Contact Person | simona.baroni@unito.it |
In Vivo Experimental Parameters
| Number Of Groups | 2 |
|---|---|
| Types Of Groups | control and monensin (16 mg/Kg) treated groups |
| Overall Sample Size | 8 |
| Disease Model | cancer |
| Organ Or Tissue | hepatocellular carcinoma |
| Sample Size For Each Group | 4,4 |
| Inclusion Criteria | none |
| Exclusion Criteria | none |
| Randomization | mice were randomly divided into treted and control group |
| Blinding | no |
| Outcome Measures | Sodium tissue content |
| Statistical Methods | t-test |
| Species | Mice |
| Strain | NSG (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) |
| Immune Status | extremely immunodeficient |
| Sex | male |
| Age | Six-to-eight-week-old |
| Weight | about 25 g |
| Genotype | PrkdcscidIl2rgtm1Wjl |
| Source Of Animals | The Jackson lab |
| Registry Number Of Animal Authorization | 851/2020-PR |
Experimental Procedures
| Pharmacological Procedures Intervention And Control | yes |
|---|---|
| Pharmacological Drug | Monensin |
| Company | Merck |
| Formulation | solution from powder |
| Drug Dose | 16 mg/Kg |
| Volume | about 0.1 ml |
| Site Route Of Administration | intraperitoneal |
| Frequency Of Administration | daily |
| Vehicle Or Carrier Solution Formulation | dilusion of the stock solution (DMSO/2-Hydroxypropyl)-b-cyclodextrin 10%) in saline |
| Blood Sampling | no |
| Surgical Procedures Including Sham Surgery | no |
| Target Organ Tissue | tumor |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | intramuscolar |
| Anesthesia Drugs | Zoletil/Rompun |
| Anesthesia Dose | 20 mg/kg tiletamine/zolazepam (Zoletil 100; Virbac, Milan, Italy) and 5 mg/kg xylazine (Rompun; Bayer, Milan, Italy). |
| Euthanasia | yes |
| Method | cervival dislocation |
| Histology | no |
| Frequency Of Imaging | daily/every 2 days |
| Timing Of Imaging | 2 h after treatment |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | no |
| Cell Line | HepaHepa1c1c7 (C1C7) hepatoma cells |
| Provenance | ECACC (Salisbury, UK) |
| Modified Cell Line | no |
| Cell Injection Route | subcutaneous |
| Number Of Cells | 1x10^6 |
Image Acquisition
| Instrument Vendor | Bruker |
|---|---|
| Instrument Type | Pharmascan 70/16, equipped with a 1H/23Na transmit-receive surface coil (20 mm diameter) |
| Instrument Specifics | 7 T |
| Image Acquisition Parameters | Proton T2-weighted images were collected using a Turbo RARE pulse sequence (FOV 30x30, MTX 128x128, slice thickness 3 mm, spatial resolution 0.23 x 0.23 mm2, TE 55 ms, TR 2500 ms, RF 12, average 1). The Na+ gradient-echo FLASH sequence was set with the following parameters: FOV 30x30, MTX 32x32, slice thickness 3 mm, spatial resolution 0.94 x 0.94 mm2, TE 2.05 ms, TR 50 ms, average 548. |
| Correction | no |
Image Correlation
| Image Type | Primary image |
|---|---|
| Format Compression | raw; DICOM |
| Dimensions | 2D |
| Field Of View | 30x30 |
| Dimension Extents | 1H 128x128, 23Na 32x32 |
| Size Description | 1H 128x128x3 mm, 23Na 32x32x3 mm |
| Image Processing Methods | default reconstruction |
Analyzed Data
| Analysis Result Type | numerical |
|---|---|
| Data Used For Analysis | ROI, Na standard reference |
| Analysis Method And Details | Manual delineation of tumor regions |
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C16809 |
|---|---|
| Ncit Imaging Submodality | NCIT:C154542,NCIT:C181740,NCIT:C180729 |
| Doid | DOID:684 |
| Ncit Anatomy | NCIT:C3099 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C15166 |
| Chebi Pharmaco | CHEBI:27617 |
| Chebi Anesthesia | CHEBI:92386 |