Metadata
Dataset ID:
15
Study Design
| Study Design Background | Development of metrics for improving tumor pH quantification in presence of fat |
|---|---|
| Study Description | CEST tumor pH fat correction |
| Study Type | preclinical |
| Study Subtype | in vitro / in vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | Development and Validation of Four Different Methods to Improve MRI-CEST Tumor pH Mapping in Presence of Fat |
| Paper Authors | Gammaraccio F, Villano D, Irrera P, Anemone AA, Carella A, Corrado A, Longo DL |
| Affiliation | Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy; Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; |
| Paper Journal | Journal of Imaging |
| Paper Year | 2024 |
| Paper Doi | https://doi.org/10.3390/jimaging10070166 |
| Open Access | yes |
| Updated Year | 2024 |
Study Component
| Multi Modality Images | no |
|---|---|
| Imaging Modality | MRI |
| Imaging Sub Modality | CEST |
| Radiation | no |
| Imaging Coverage | abdomen |
| Imaging Target | tumor |
Dataset Information
| Institution | CNR-IBB |
|---|---|
| Pi | Dario Longo |
| Country Of Institution | Italy |
| Imaging Facility | CNR-IBB Torino |
| Euro Bio Imaging Node | MMMI |
| Country Of Imaging Facility | Italy |
| Funding | yes |
| Funding Agency | Associazione Italiana Ricerca sul Cancer (AIRC) |
| Grant Number | MFAG 2017 #20153 |
| Dataset Access | public |
| License | CC BY |
| Duo Data Use Permission | DUO:0000042 |
| Duo Data Use Modifier | DUO:0000019, DUO:0000046 |
| Duo Investigation | DUO:0000037, DUO:0000040, DUO:0000031 |
| Contact Person | dariolivio.longo@cnr.it |
| Link To Dataset1 | https://eubi-xnat.hpc4ai.unito.it/app/template/XDATScreen_report_xnat_projectData.vm/search_element/xnat:projectData/search_field/xnat:projectData.ID/search_value/FatCorrectionDataset |
In Vivo Experimental Parameters
| Number Of Groups | 2 |
|---|---|
| Types Of Groups | low fat, high fat |
| Overall Sample Size | 2 |
| Disease Model | cancer |
| Organ Or Tissue | mammary gland |
| Sample Size For Each Group | 1.1 |
| Randomization | no |
| Procedures To Keep Treatments Blind | no |
| Outcome Measures | CEST contrast, tumor pH |
| Species | Mice |
| Strain | Balb/C, MMTV-PyMT |
| Immune Status | immunecompetent |
| Sex | female, female |
| Age | 7 weeks, 8 weeks |
| Weight | 25 grams |
| Genotype | heterozygous |
| Genetic Manipulation | transgenic |
| Source Of Animals | Charles River Laboratories Italia srl, Calco Italy; The Jackson Laboratory, Bar Harbor, ME, USA |
Experimental Procedures
| Pharmacological Procedures Intervention And Control | no |
|---|---|
| Blood Sampling | no |
| Surgical Procedures Including Sham Surgery | no |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | intramuscular |
| Duration | 40 |
| Anesthesia Drugs | xylazine (Rompun, Bayer, Italy), tiletamine/zolazepam (Zoletil 100, Virbac, Italy) |
| Anesthesia Dose | 5 mg/kg, 20 mg/kg |
| Euthanasia | yes |
| Method | cervical dislocation |
| Histology | no |
| Frequency Of Imaging | single scan |
| Overall Scan Length | 40 minutes |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | yes |
| Contrast Agent Commercial Drug | Isovue |
| Contrast Agent Chemical Drug | Iopamidol |
| Contrast Agent Dose | 4 g/kg |
| Injection Volume | 0.25 |
| Route Of Administration | intravenous |
| Cell Line | 4T1 |
| Provenance | Molecular Biotechnology Center Cell Factory |
| Modified Cell Line | no |
| Cell Injection Route | subcutaneous |
| Number Of Cells | 1000000 |
| Housing Room | not SPF |
| Dietary Intervention | no |
| Respiration Rate | no |
| Body Tempurature Etc | no |
| Food Intake Measured | no |
Image Acquisition
| Instrument Vendor | Bruker |
|---|---|
| Instrument Type | Microimaging |
| Instrument Specifics | 7T |
| Raw Data | yes |
| Qa Qc | no |
Image Correlation
| Image Type | primary image/processed image |
|---|---|
| Image Scale | signal intensity |
| Format Compression | DICOM |
| Dimensions | 4D (3D + frequencies) |
| Overall Number Of Images | 16 |
| Field Of View | 30x30x1.5 mm |
| Dimension Extents | 128x128x8 |
| Size Description | 30x30x12 mm |
| Pixel Voxel Size Description | 0,234x0,234x1,5 mm |
| Image Processing Methods | default reconstruction |
| Image Reconstruction Algorithm | n/a |
| Quality Control | no |
| Image Smoothing Or Filtering Algorithm | n/a |
| Image Registration Algorithm | no |
| Ai Enhanced Algorithm | yes |
| Qc Info | R2 calculation on raw CEST data |
Analyzed Data
| Analysis Result Type | numerical analysis |
|---|---|
| Data Used For Analysis | pH maps |
| Analysis Method And Details | manual delineation of tumor regions (ROI), MATLAB |
| File Format Of Result File Csv Json Txt Xlsx | jpg, txt, mat |
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C16809 |
|---|---|
| Ncit Imaging Submodality | NCIT:C116451 |
| Doid | DOID:1612,DOID:162 |
| Ncit Anatomy | NCIT:C12367 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C37357 |
| Chebi Anesthesia | CHEBI:92386,CHEBI:6121 |
| Chebi Contrast Agent Commercial Name | CHEBI:31711 |
| Chebi Contrast Agent Chemical Name | CHEBI:31711 |
| Clo | CLO:0001401 |