Metadata
Dataset ID:
19
Study Design
| Study Design Background | This preclinical study is aimed at developing and evaluating a novel radiotracer for HER2-targeted therapy selection and monitoring. The research focuses on the design, synthesis and characterization of a new Mn(II) chelator, BPPA, which is conjugated to trastuzumab and labeled with [52Mn]Mn isotope |
|---|---|
| Study Description | Evaluation of [52 Mn]Mn-BPPA-Trastuzumab |
| Study Type | preclinical2 |
| Study Subtype | in vivo, ex vivo |
Publication
| Paper Linked | no |
|---|---|
| Paper Title | [52 Mn]Mn-BPPA-Trastuzumab: A Promising HER2-Specific PET Radiotracer |
| Paper Authors | Toàn Ngô Minh, Vágner Adrienn, Nagy Gábor, Ország Gábor, Nagy Tamás, Csikos Csaba, Váradi Balázs, Sajtos Gergo, Kapus István, Szoboszlai Zoltán, Szikra Dezso, Trencsényi György, Tircsó Gyula, Garai Ildiko |
| Affiliation | Medical Imaging Clinic, Clinical Centre, University of Debrecen; Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen; Scanomed Ltd; Department of Physical Chemistry, Institute of Chemistry, Faculty of Science and Technology, University of Debrecen; Doctoral School of Chemistry, Faculty of Science and Technology, University of Debrecen |
| Paper Journal | Journal of Medicinal Chemistry |
| Paper Year | 2024 |
| Paper Doi | https://doi.org/10.1021/acs.jmedchem.4c00344 |
| Open Access | no |
| Updated Year | 2025 |
Study Component
| Multi Modality Images | yes |
|---|---|
| Imaging Modality | PET/MRI |
| Imaging Sub Modality | T2w |
| Radiation | yes |
| Imaging Coverage | whole body |
| Imaging Target | receptor expression |
Dataset Information
| Institution | University of Debrecen |
|---|---|
| Pi | Ildikó Garai |
| Co Pi | Gyula Tircsó |
| Country Of Institution | Hungary |
| Imaging Facility | Scanomed Ltd. |
| Country Of Imaging Facility | Hungary |
| Funding | yes |
| Funding Agency | Gedeon Richter Ph.D. Fellowship; Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund |
| Grant Number | K-134694 |
| Dataset Access | public |
| License | CC BY |
| Duo Data Use Permission | DUO:0000042 |
| Duo Data Use Modifier | DUO:0000012 |
| Duo Investigation | DUO:0000039 |
| Contact Person | arato.viktoria@med.unideb.hu |
In Vivo Experimental Parameters
| Number Of Groups | 7 |
|---|---|
| Types Of Groups | untreated, treated , treated2, treated3, treated4 |
| Overall Sample Size | 20 |
| Disease Model | cancer |
| Organ Or Tissue | mammary gland |
| Sample Size For Each Group | untreated 8, treated1 3, treated2 3, treated3 3, treated4 3 |
| Randomization | no |
| Blinding | no |
| Species | mice |
| Strain | Balb/c and SCID |
| Immune Status | immunecompetent, immunedefficient |
| Sex | female |
| Age | 12 weeks old |
| Weight | 19.5 ± 0.5 grams |
| Genotype | n/a |
| Genetic Manipulation | n/a |
| Gene | n/a |
| Source Of Animals | Charles River Laboratories |
Experimental Procedures
| Blood Sampling | yes |
|---|---|
| Blood Timing | once |
| Surgical Procedures Including Sham Surgery | no (only tumor injection) |
| Target Organ Tissue | mammary gland |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia For Imaging | yes |
| Anesthesia Type | gas |
| Anesthesia Drugs | Forane |
| Anesthesia Dose | 1,5% in 400ml/min oxygen |
| Monitoring Regime | respiratoy rate, cardiac rate |
| Euthanasia | yes |
| Method | euthanized with 5 % Forane |
| Histology | yes |
| Tissues Collected Post Euthanasia | mammary gland tissue |
| Timing Of Collection | at euthanasia |
| Imaging | yes |
| Frequency Of Imaging | one-day experiment |
| Timing Of Imaging | 7 days after the tumor cell inoculation |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | yes |
| Contrast Agent Commercial Drug | n/a |
| Contrast Agent Chemical Drug | [52Mn]Mn-DOTA-GA, [52Mn]Mn-BPPA, [52Mn]MnCl2 |
| Contrast Agent Dose | 2.4±0.4 MBq |
| Injection Volume | depends on the radioactive concentration |
| Route Of Administration | intravenous |
| Cell Lines | yes |
| Cell Line | 4T1, MDA-MB-HER2+ |
| Provenance | American Type Culture Collection (ATCC) |
| Housing Room | SPF |
Image Acquisition
| Instrument Type | Mediso Ltd. |
|---|---|
| Instrument Specifics | NanoScan PET/MRI |
| Image Acquisition Parameters | 3T, FOV: 32 mm × 32 mm |
| Raw Data | decay correction, attenuation correction |
| Qa Qc | yes |
Image Correlation
| Image Type | processed image |
|---|---|
| Image Scale | SUV |
| Format Compression | DICOM |
| Dimensions | 2D, 3D |
| Field Of View | Bore size: 16 cm, Transaxial FOV: 12 cm, Axial FOV: 10 cm |
| Dimension Extents | 296, 296, 35 |
| Size Description | 296*0, 2694, 296*0, 2694, 35*1, 347 |
| Pixel Voxel Size Description | 1.12 mm×1.12 mm×13 mm |
| Image Processing Methods | reconstruction, smoothing |
| Image Reconstruction Algorithm | OSEM-LOR Spatial resolution with Tera-Tomo (3D OSEM): 0.7 mm, Spatial Resolution with FBP (NEMA): 1.25 mm |
| Quality Control | smoothing factor of 0.02 |
| Image Smoothing Or Filtering Algorithm | BrainCad |
| Image Registration Algorithm | AI denoising |
| Ai Enhanced Algorithm | yes |
Analyzed Data
| Analysis Method And Details | SUV |
|---|---|
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C103514 |
|---|---|
| Ncit Imaging Submodality | NCIT:C180729 |
| Doid | DOID:1612 |
| Ncit Anatomy | NCIT:C12367 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C37357,NCIT:C15159 |
| Chebi Anesthesia | CHEBI:6015 |
| Clo | CLO:0001401 |