Metadata
Dataset ID:
2
Study Design
| Study Design Background | Improvement of external x-ray beam radiation therapy of breast cancer by injecting barium based nano-particles. In-vivo test in a mouse breast cancer model. 30uL of Particles were injected intra-tumoraly after the tumor had overcome a size of 300 mm3. The tumor area was irradiated 10 times with about 1 Gy per session. 2 days after the last irradiation mice were sacrificed and the tumors explanted. Tumors have been formalin fixed and embedded in paraffin. Aim of the Synchrotron study was to localize the particles and overlay that with subsequently perform histological analysis. |
|---|---|
| Study Description | Therakon |
| Study Type | preclinical |
| Study Subtype | ex-vivo |
Publication
| Paper Linked | In preparation |
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| Updated Year | 2022 |
Study Component
| Multi Modality Images | not |
|---|---|
| Imaging Modality | CT |
| Imaging Sub Modality | Phase-contrast CT |
| Radiation | yes |
| Imaging Coverage | Mammary gland |
| Imaging Target | Anatomy |
Dataset Information
| Institution | University Medical Center Goettingen |
|---|---|
| Pi | Christian Dullin |
| Country Of Institution | Germany |
| Imaging Facility | Elettra sincrotrone Trieste |
| Euro Bio Imaging Node | Phase Contrast Imaging Flagship Node |
| Country Of Imaging Facility | Italy |
In Vivo Experimental Parameters
| Number Of Groups | 3 |
|---|---|
| Types Of Groups | pure radiation therapy, radiation therapy plus nanoparticles, untreated animals |
| Overall Sample Size | 16 |
| Disease Model | breast cancer |
| Organ Or Tissue | mammary gland |
| Sample Size For Each Group | 4 for pure radiation therapy, 4 for radiation therapy plus nanoparticles, 8 for control group |
| Power Calculation | none |
| Randomization | yes mice were randomly assigned to the three groups,trying to maintain the same distribution of tumor sizes and growth rates in all groups |
| Blinding | no |
| Outcome Measures | Relative tumor growth, weight loss, general well being of the mice |
| Statistical Methods | ANOVA |
| Species | Mice |
| Strain | Balb/c |
| Immune Status | Immunecompetent |
| Sex | Female |
| Age | Older than 6 weeks |
| Age At Start Experiment | Older than 6 weeks |
| Age At Scanning Experiment S | Older than 9 weeks (no scanning - irradiation) |
| Weight | About 20 g |
| Weight At Start Experiment | About 20 g |
| Weight At End Experiment | About 20 g |
| Genetic Manipulation | KO |
| Gene | Wap-Tag |
| Source Of Animals | Own breeding colony MPI for multisciplinary sciences Goettingen Germany |
| Registry Number Of Animal Authorization | 33.9-42502-04-18/3022 |
Experimental Procedures
| Pharmacological Drug | Barium based nano-particles diameter 120nm (experimental) |
|---|---|
| Company | NanoPet Pharma Berlin Germany |
| Formulation | No disclosure |
| Drug Dose | 450 |
| Volume | 0,03 |
| Concentration | 300 mg/ml |
| Site Route Of Administration | Intratumoral |
| Frequency Of Administration | One time |
| Vehicle Or Carrier Solution Formulation | PBS |
| Blood Sampling | no |
| Surgical Procedures Including Sham Surgery | yes |
| Description Of The Surgical Procedure | Implantation of 1Mio tumor cells in the mammary gland |
| Target Organ Tissue | Mammary gland |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | Gas |
| Duration | About 5 |
| Anesthesia Drugs | Isofluran |
| Anesthesia Dose | 2% in 1L per minute O2/air mix |
| Monitoring Regime | Visual surveillance |
| Euthanasia | yes |
| Method | CO2 plus cervical dislocation |
| Histology | yes |
| Tissues Collected Post Euthanasia | Tumor, lung,heart, liver, spleen |
| Timing Of Collection | 2 days after last irradiadtion |
| Histological Procedure | Fixed (formalin) |
| Length Of Fixation | 24 hours |
| Frequency Of Imaging | Single event |
| Timing Of Imaging | After fixation |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | yes |
| Contrast Agent Commercial Drug | Experimental contrast agent |
| Contrast Agent Chemical Drug | Experimental contrast agent |
| Contrast Agent Dose | 0,45 g/kg |
| Injection Volume | 0,03 |
| Route Of Administration | Intratumoral |
| Cell Line | pH8N8 |
| Provenance | Institute for Tumor Biology, University Medical Center Hamburg-Eppendorf,Hamburg, Germany |
Image Acquisition
| Instrument Vendor | Custom Made |
|---|---|
| Instrument Type | X-Ray beamline |
| Instrument Specifics | Free space propagation-based phase contrast imaging with white beam |
| Image Acquisition Parameters | White beam with 0.5 mm Si filtration, mean beam energy of 16.7 keV |
| Correction | Phase retrieval |
| Raw Data | yes |
Image Correlation
| Image Type | processed (tomographic reconstruction) |
|---|---|
| Image Scale | linear attenuation coefficient ? (1/cm) |
| Dimensions | 3D |
| Overall Number Of Images | 1 |
| Field Of View | 7.6 mm x 4mm |
| Dimension Extents | 3800x2048x2048 |
| Size Description | 7.6 mm x 4 mm x 4 mm |
| Pixel Voxel Size Description | 2 um x 2 um x 2 um |
| Image Processing Methods | Phase retrieval |
| Image Reconstruction Algorithm | FBP (ram-lak) |
| Ai Enhanced Algorithm | no |
Analyzed Data
| Status | ongoing |
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Ontology Terms
| Ncit Imaging | NCIT:C17204 |
|---|---|
| Ncit Imaging Submodality | NCIT:C137805 |
| Doid | DOID:1612 |
| Ncit Anatomy | NCIT:C12367 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C37357 |
| Chebi Pharmaco | CHEBI:32594 |
| Chebi Anesthesia | CHEBI:6015 |