Metadata
Dataset ID:
22
Study Design
| Study Design Background | Sudy of rare disease (aceruplasmonemia) using Cp KO mouse model to highlight gender differences in disease behaviour in view of a possible replacement therapy. Labeling of Ceruloplasmine (Cp) with Cu-64 to follow its in vivo distribution in the model. Study of inflammation. |
|---|---|
| Study Description | Ceruloplasmin administration in the preclinical mouse model of aceruloplasminemia reveals a sex-related variation in biodistribution |
| Study Type | preclinical |
| Study Subtype | in vivo and post mortem |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | Ceruloplasmin administration in the preclinical mouse model of aceruloplasminemia reveals a sex-related variation in biodistribution |
| Paper Authors | Sara Belloli, Cristina Monterisi, Paolo Rainone, AngelaColiva, Alan Zanardi, Antonio Conti, Andrea Caricasole, Rosa Maria Moresco & Massimo Alessio |
| Affiliation | 1. Nuclear Medicine and PET Cyclotron Unit, IRCCS-Ospedale San Raffaele, Milano, Italy. 2. Institute of Bioimaging and Complex Biological Systems, CNR, Segrate, MI, Italy. 3. Medicine and Surgery Department, University of Milano - Bicocca, Monza, MB, Italy. 4. Proteome Biochemistry, COSR-Center for Omics Sciences, IRCCS-Ospedale San Raffaele, Milano, Italy. 5. Research and Innovation, Kedrion S.p.A., Loc. Bolognana, Gallicano, LU, Italy. 6. Proteome Biochemistry, COSR-Center for Omics Sciences, IRCCS-Ospedale San Raffaele, Milano, Italy. |
| Paper Journal | Communications biology |
| Paper Year | 2025 |
| Paper Doi | https://doi.org/10.1038/s42003-025-07714-8 |
| Open Access | yes |
Study Component
| Multi Modality Images | no |
|---|---|
| Imaging Modality | PET/CT |
| Radiation | yes |
| Imaging Coverage | whole body |
| Imaging Target | Copper transporter, TSPO receptor |
Dataset Information
| Institution | IBSBC-CNR |
|---|---|
| Pi | Massimo Alessio |
| Co Pi | Rosa Maria Moresco |
| Country Of Institution | Italy |
| Imaging Facility | Laboratory of Preclinical Molecular Imaging |
| Euro Bio Imaging Node | Multi-Modal Molecular Imaging Italian node (MMMI) |
| Country Of Imaging Facility | Italy |
| Funding | yes |
| Funding Agency | Ricerca Finalizzata 2018, Italian Ministry ofHealth (RF-2018-12366471), Eu-BI MMMI FOE 2021, PNRR See Life Project IR0000023 |
| Dataset Access | public |
| License | CC BY-NC-ND 4.0 |
| Duo Data Use Permission | DUO:0000042 |
| Duo Data Use Modifier | DUO:0000020 |
| Duo Investigation | DUO:0000032 |
| Contact Person | sara.belloli@cnr.it; alessio.massimo@hsr.it |
In Vivo Experimental Parameters
| Number Of Groups | 8 |
|---|---|
| Types Of Groups | female adult wt, female wt aged, female adult KO, female aged KO, male adult wt, male wt aged, male adult KO, male aged KO |
| Overall Sample Size | 48 |
| Disease Model | rare disease with neurological signs and inflammation |
| Organ Or Tissue | brain and liver |
| Sample Size For Each Group | 6 per each group |
| Power Calculation | G*Power |
| Randomization | no |
| Blinding | no |
| Species | Mice |
| Strain | C57BL/6 |
| Immune Status | immunocompetent |
| Sex | female and male |
| Age | adult and aged |
| Age At Start Experiment | 6 months |
| Weight | 40 g |
| Genotype | homozygous |
| Genetic Manipulation | Cp KO |
| Gene | Ceruloplasmine |
Experimental Procedures
| Pharmacological Procedures Intervention And Control | no |
|---|---|
| Blood Sampling | yes |
| Blood Sampling Method | cardiac puncture before sacrifice |
| Blood Sample Volume | 500 microliter |
| Blood Timing | one |
| Surgical Procedures Including Sham Surgery | no |
| Target Organ Tissue | brain, eyes, liver, small intestine, lung, kidney, heart and chest muscle |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia For Imaging | yes |
| Anesthesia Type | gas |
| Duration | 30 minutes |
| Anesthesia Drugs | isoflurane |
| Euthanasia | yes |
| Method | cardiac puncture and cervical dislocation |
| Histology | yes |
| Tissues Collected Post Euthanasia | brain, eyes, liver, small intestine, lung, kidney, heart and chest muscle |
| Timing Of Collection | end of imaging |
| Imaging | yes |
| Frequency Of Imaging | one per group |
| Timing Of Imaging | selected age of the Cp KO animals |
| Overall Scan Length | 30 minutes |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | yes |
| Contrast Agent Commercial Drug | [64Cu]-Ceruloplasmine; [18F]-VC701 |
| Contrast Agent Chemical Drug | [64Cu]-Ceruloplasmine; [18F]-3-Fluoromethyl-N-methyl-4-phenyl-N-(phenylmethyl) quinoline-2-carboxamide |
| Contrast Agent Dose | 5.12 ± 0.25 MBq; 1.91 ± 0.27MBq |
| Injection Volume | 50ul |
| Route Of Administration | intravenous |
| Cell Lines | no |
Image Acquisition
| Instrument Vendor | Molecubes |
|---|---|
| Instrument Type | β-cube, X-cube |
| Instrument Specifics | PET: FOV of 72x130 mm; CT: FOV of 64x40 mm |
| Image Acquisition Parameters | PET: 511 KeV, 30% energy resolution; CT: 40 kVp, 400 μA (0.8 mm Al filtration) |
| Correction | PET: isotope decay and random coincidences correction; CT: ring artifact correction |
| Raw Data | yes |
| Qa Qc | yes |
Image Correlation
| Image Type | Reconstructed and quantified images |
|---|---|
| Image Scale | KBq/cc for PET and HU for CT |
| Format Compression | raw, DICOM and NIFTI |
| Dimensions | 2D/3D |
| Overall Number Of Images | 1 dataset per animal per imaging technique per time point |
| Field Of View | Max FOV for PET and CT |
| Dimension Extents | PET/CT: 192x192x384 |
| Size Description | PET/CT: 76.8 x 76.8 x 154 mm |
| Pixel Voxel Size Description | PET/CT: 400 µm isometric |
| Image Processing Methods | CT-based normalization |
| Image Reconstruction Algorithm | PET: OSEM (30 iterations); CT: ISRA (iterative) |
| Quality Control | no |
Analyzed Data
| Data Used For Analysis | SUVmean |
|---|