Metadata
Dataset ID:
3
Study Design
| Study Design Background | Study of the effectiveness of two different gamma-irradiated bone graft materials for bone regeneration in rat femurs. Ex vivo imaging was conducted at three time points after surgical induction of femur defect (drill hole defect, 3 mm diameter at mid-diaphysis), followed by defect filling with two types of composites. In vivo micro-CT imaging was also conducted after surgery and before each study time point (before euthanasia) with the goal of qualitatively monitoring the bone healing process on a macroscopic scale |
|---|---|
| Study Description | TissueLab |
| Study Type | preclinical |
| Study Subtype | ex-vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | Evaluation of the Effect of a Gamma Irradiated DBM-Pluronic F127 Composite on Bone Regeneration in Wistar Rat |
| Paper Authors | Al Kayal, Tamer, Panetta, Daniele, Canciani, Barbara, Losi, Paola, Tripodi, Maria, Burchielli, Silvia, Ottoni, Priscilla, Salvadori, Piero Antonio, Soldani, Giorgio |
| Affiliation | Institute of Clinical Physiology- CNR, Pisa, Italy, University & IRCCS AOU San Martino—IST, National Institute for Cancer Research, DIMES, Genova, Italy, CNR/Tuscany “G. Monasterio” Foundation, Pisa Italy |
| Paper Journal | Plos ONE |
| Paper Year | 2015 |
| Paper Doi | https://doi.org/10.1371/journal.pone.0125110 |
| Open Access | yes |
| Updated Year | 2022 |
Study Component
| Multi Modality Images | No |
|---|---|
| Imaging Modality | CT |
| Radiation | yes |
| Imaging Coverage | Femur |
| Imaging Target | Bone healing |
Dataset Information
| Institution | CNR IFC, Pisa |
|---|---|
| Pi | Daniele Panetta |
| Country Of Institution | Italy |
| Imaging Facility | CNR IFC, Pisa |
| Euro Bio Imaging Node | MMMI Italy |
| Country Of Imaging Facility | Italy |
In Vivo Experimental Parameters
| Number Of Groups | 2 |
|---|---|
| Types Of Groups | Graft materials: demineralized bone matrix (DBM) + Pluronic(TM) F127 (group 1), Pluronic F127 only (group 2) |
| Overall Sample Size | 36 |
| Disease Model | bone disease |
| Organ Or Tissue | femur |
| Sample Size For Each Group | 18 rats per type of graft material. On each group, 3 subgroups of 6 animals each were used to test three different time points (15, 30 and 90 days from surgery) |
| Randomization | yes |
| Blinding | no |
| Outcome Measures | Bone volume (BV) and bone surface to bone volume ratio (BS/BV) inside three different volumes of interest (VOI) centered on the drill hole defects |
| Statistical Methods | t-test, 2-tails (group 1 vs group 2 at each time point) |
| Species | Rats |
| Strain | Wistar |
| Sex | Male |
| Age | 3 months |
| Age At Start Experiment | 3 months |
| Weight | ca. 300 g |
| Source Of Animals | Harlan Laboratories S.r.l. (Udine, Italy) |
| Registry Number Of Animal Authorization | 250/2011-B |
Experimental Procedures
| Pharmacological Drug | 20% (w/v) solution of Pluronic F127 in saline solution; DBM-PF127 composite, prepared mixing DBM particles (<80 um) and PF127 solution at 4°C to obtain a composite containing 40% (w/w) DBM |
|---|---|
| Company | TissueLab srl (DBM); Sigma-Aldrich, MO, USA (PF127) |
| Volume | 0,005 |
| Site Route Of Administration | Local, on bone defect (one side only, the contralateral femur is kept empty for control) |
| Frequency Of Administration | Once, at the time of surgery |
| Blood Sampling | no |
| Surgical Procedures Including Sham Surgery | yes |
| Description Of The Surgical Procedure | Drilling hole defect on each femur, mid-diaphysis, 3 mm diameter |
| Target Organ Tissue | Femur |
| Pathogen Infection Intervention And Control | no |
| Anesthesia Type | Intraperitoneal |
| Duration | 3 |
| Anesthesia Drugs | Zoletil+Xilazine |
| Monitoring Regime | Visual surveillance |
| Euthanasia | yes |
| Method | Isoflurance inhalation |
| Histology | yes |
| Tissues Collected Post Euthanasia | Femurs |
| Timing Of Collection | At each time point after euthanasia (15, 30 and 90 days post-surgery) |
| Histological Procedure | Fixed (formalin) |
| Frequency Of Imaging | In vivo (qualitative monitoring only): two times (post-surgery and at selected time point). Ex-vivo: single event |
| Timing Of Imaging | After fixation |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | no |
Image Acquisition
| Instrument Vendor | Custom made |
|---|---|
| Instrument Type | XALT-HR micro-CT |
| Instrument Specifics | Variable-magnification micro-CT operating in cone-beam geometry |
| Image Acquisition Parameters | 50 kV, 0.7 mA, 1 mm Al filtration |
| Correction | Dark signal, flat-field equalization, ring artifact suppression |
| Raw Data | yes |
Image Correlation
| Image Type | Primary image (CT) |
|---|---|
| Image Scale | Hounfield Units |
| Format Compression | Analyze 7.5 |
| Dimensions | 3D |
| Overall Number Of Images | 1 |
| Field Of View | 18 mm x 30 mm (transaxial x axial) |
| Dimension Extents | 700x700x1300 |
| Size Description | 13 mm x 13 mm x 24 mm |
| Pixel Voxel Size Description | 18,4 um x 18,4 um x 18,4 um |
| Image Reconstruction Algorithm | FBP (ram-lak) |
| Ai Enhanced Algorithm | no |
Analyzed Data
| Status | complete |
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Ontology Terms
| Ncit Imaging | NCIT:C17204 |
|---|---|
| Doid | DOID:0080001 |
| Ncit Anatomy | NCIT:C12717 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C76190 |
| Chebi Pharmaco | CHEBI:53514 |
| Chebi Anesthesia | CHEBI:92386 |