Metadata
Dataset ID:
4
Study Design
| Study Design Background | Study of the causal role of the gut microbiota on cognitive development and cardiometabolic health. Germ-free mice were transplanted by oral gavage with fecal material obtained from 5 year old children, with gender coherence between donor and recipients. Mice body growth, food intake and cognitive function ware monitored for 5 months after fecal material transplantation (FMT). Then, total body positron emission tomography (microPET) coupled with FDG, and computed tomography (microCT) scans were run to measure glucose metabolism and radiodensity in several organs, with focus on brain, adipose tissues, myocardium, liver, gut. Ultrasound was also applied to measure cardiac morphological and functional parameters, and to assess hepatic lipid content. |
|---|---|
| Study Description | GUTMOM |
| Study Type | preclinical |
| Study Subtype | in vivo and ex vivo |
Publication
| Paper Linked | in preparation |
|---|---|
| Updated Year | 2022 |
Study Component
| Multi Modality Images | yes |
|---|---|
| Imaging Modality | PET,CT,US |
| Imaging Coverage | "PET/CT total body; US heart and liver" |
| Imaging Target | Glucose metabolism (PET), anatomy (CT), lipid content (US, CT), cardiac function (US) |
Dataset Information
| Institution | CNR IFC, Pisa |
|---|---|
| Pi | Patricia Iozzo |
| Co Pi | Maria Angela Guzzardi |
| Country Of Institution | Italy |
| Imaging Facility | CNR IFC, Pisa |
| Euro Bio Imaging Node | MMMI Italy |
| Country Of Imaging Facility | Italy |
In Vivo Experimental Parameters
| Number Of Groups | 4 |
|---|---|
| Types Of Groups | male transplanted, female transplanted, male sham, female sham |
| Overall Sample Size | 72 |
| Disease Model | disease of metabolism |
| Organ Or Tissue | brain, adipose tissue, liver, myocardium, gut |
| Sample Size For Each Group | 30 males transplanted, 30 females transplanted, 6 males sham, 6 females sham |
| Randomization | yes, mice were randomly assigned to groups |
| Blinding | no |
| Outcome Measures | "FDG fractional extraction and glucose metabolism of brain, adipose tissue, liver, myocardium, gut; tissues radiodensity, adipose tissue volumes, hepatic lipid content, cardiac function" |
| Statistical Methods | one-way ANOVA, 2-tails t-test |
| Species | Mice |
| Strain | C57BL/6 |
| Sex | males and females (50/50%) |
| Age | 4-5 weeks |
| Age At Start Experiment | 4-5 weeks |
| Age At Scanning Experiment S | About 6 months |
| Weight At Start Experiment | About 20 g |
| Weight At End Experiment | About 30 g |
| Source Of Animals | Charles River Laboratories s.r.l. (Milano, Italy) |
| Registry Number Of Animal Authorization | 464/2018-PR |
Experimental Procedures
| Pharmacological Drug | fecal material, prepared in PBS (100 mg of stool in 0.77 ml if sterile PBS, vortex and filterd). The filtrated was adminsterd |
|---|---|
| Volume | 0,15 |
| Site Route Of Administration | oral gavage |
| Frequency Of Administration | once, at the beginnig of the protocol |
| Vehicle Or Carrier Solution Formulation | PBS |
| Blood Sampling | yes |
| Blood Sample Volume | About 300 ml |
| Blood Timing | Once, after the animal was sacrificed |
| Surgical Procedures Including Sham Surgery | no |
| Anesthesia Type | gas |
| Duration | 100 |
| Anesthesia Drugs | isoflurane |
| Anesthesia Dose | 1-2% |
| Monitoring Regime | visual surveillance and cardiac rate |
| Euthanasia | yes |
| Method | isoflurane overdose |
| Histology | yes |
| Tissues Collected Post Euthanasia | All organs |
| Timing Of Collection | immediately after PET-CT imaging |
| Histological Procedure | Fixed (formalin) |
| Frequency Of Imaging | Single event |
| Timing Of Imaging | 5 months after fecal material transplantation, at the end of the in vivo protocol |
| Overall Scan Length | 100 |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | yes |
| Contrast Agent Chemical Drug | 18F-fluorodeoxyglucose (FDG) |
| Contrast Agent Dose | 6.6 MBq |
| Injection Volume | 0,26 |
| Route Of Administration | intraperitoneal |
Analyzed Data
| Status | ongoing |
|---|
Ontology Terms
| Ncit Imaging | NCIT:C17204,NCIT:C17230 |
|---|---|
| Doid | DOID:0014667 |
| Ncit Anatomy | NCIT:C12439,NCIT:C12472,NCIT:C12392,NCIT:C34082,NCIT:C12371 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C14424 |
| Chebi Anesthesia | CHEBI:6015 |
| Chebi Contrast Agent Chemical Name | CHEBI:49134 |