Metadata
Dataset ID:
7
Study Design
| Study Design Background | Inflammation assessment by in vivo PET and MRI of a Parkinson's disease model (MPTP) using the TSPO specific radiotracer [18F]VC701. Implementation of new tool analysis for mouse brain PET fully based on CT-images |
|---|---|
| Study Description | Molecular and imaging prodromal markers of neurodegeneration in Parkinson disease |
| Study Type | preclinical |
| Study Subtype | in vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | Development of a new toolbox for mouse PET-CT brain image analysis fully based on CT images and validated in a PD mouse model |
| Paper Authors | Presotto L, Bettinardi V, Mercatelli D, Picchio M, Morari M, Moresco RM and Belloli S |
| Affiliation | IBFM-CNR, University of Milano - Bicocca, IRCCS San Raffaele of Milan, University of Ferrara, Italy |
| Paper Journal | Scientific reports |
| Paper Year | 2022 |
| Paper Doi | https://doi.org/10.1038/s41598-022-19872-4 |
| Open Access | yes |
| Updated Year | 2022 |
Study Component
| Multi Modality Images | yes |
|---|---|
| Imaging Modality | PET/CT,MRI |
| Imaging Sub Modality | T2w MRI |
| Radiation | Yes |
| Imaging Coverage | CT: head; PET: whole body |
| Imaging Target | TSPO receptor activity |
Dataset Information
| Institution | IBFM-CNR |
|---|---|
| Pi | Moresco Rosa Maria |
| Co Pi | Belloli Sara |
| Country Of Institution | Italy |
| Imaging Facility | Laboratory of molecular imaging for small animal |
| Euro Bio Imaging Node | MMMI Italy |
| Country Of Imaging Facility | Italy |
In Vivo Experimental Parameters
| Number Of Groups | 1 |
|---|---|
| Types Of Groups | pre- e post-MPTP (1-metil 4-fenil 1,2,3,6-tetraidro-piridina) in a test-retest study |
| Overall Sample Size | 6 |
| Disease Model | Parkinson's disease |
| Organ Or Tissue | brain |
| Sample Size For Each Group | 6 mice pre and post-treated with MPTP |
| Power Calculation | none, pilot study |
| Randomization | single group |
| Blinding | no |
| Statistical Methods | Student's t test for paired samples |
| Species | Mice |
| Strain | C57BL/6 |
| Immune Status | Immunocompetent |
| Sex | Male |
| Age | Three-month-old |
| Age At Start Experiment | Three-month-old |
| Age At Scanning Experiment S | Three-month-old |
| Weight | About 30 g |
| Weight At Start Experiment | About 30 g |
| Weight At End Experiment | About 30 g |
| Source Of Animals | University of Ferrara, Italy |
| Registry Number Of Animal Authorization | 237/2019-PR |
Experimental Procedures
| Pharmacological Drug | MPTP |
|---|---|
| Company | MedChemExpress |
| Formulation | solution from powder |
| Drug Dose | 30 |
| Volume | 0,3 |
| Concentration | 100 mg/ml |
| Site Route Of Administration | Intraperitoneally |
| Frequency Of Administration | daily for 7 days |
| Vehicle Or Carrier Solution Formulation | Saline |
| Drug Batch Sample Number | Lot # 27361 |
| Blood Sampling | no |
| Surgical Procedures Including Sham Surgery | no |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | Gas |
| Duration | 30 |
| Anesthesia Drugs | Isofluran |
| Anesthesia Dose | 2% in 1L per minute/air mix |
| Monitoring Regime | software remote control of the animal's vital functions (temperature, heartbeat and breathing) |
| Euthanasia | yes |
| Method | CO2 plus cervical dislocation |
| Histology | no |
| Frequency Of Imaging | Two time points |
| Timing Of Imaging | Pre and post-MPTP treatment |
| Overall Scan Length | 25 min for PET-CT and 70 min for MRI |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | Yes |
| Contrast Agent Commercial Drug | [18F]VC701 |
| Contrast Agent Chemical Drug | Fluoromethyl derivative of PK11195 |
| Contrast Agent Dose | mean injected activity: 4,7 ± 0,3 MBq |
| Injection Volume | 0,03 |
| Injection Time | scanning 100 min after radiotracer's injection |
| Vehicle | saline |
| Route Of Administration | intravenous |
Image Acquisition
| Instrument Vendor | PET-CT Molecubes; MRI Bruker |
|---|---|
| Instrument Type | β-Cube and X-Cube for PET and CT, respectively;Bruker Biospec 70/30 for MRI |
| Instrument Specifics | PET: FOV of 72x130 mm; CT: FOV of 64x40 mm; 7Tesla MRI equipped with 450/675 mT/m gradients |
| Image Acquisition Parameters | PET: 511 KeV, 30% energy resolution; CT: 40 kVp, 400 ?A (0.8 mm Al filtration) |
| Correction | PET: isotope decay and random coincidences correction; CT: ring artifact correction |
| Raw Data | Yes |
| Qa Qc | Yes. PET: daily and mothly QC; CT: monthly QC; MRI: daily QC |
Image Correlation
| Image Type | Reconstructed and quantified images |
|---|---|
| Image Scale | KBq/cc for PET and HU for CT, T2 relaxation time for MRI |
| Format Compression | raw, DICOM and NIFTI |
| Dimensions | 2D/3D |
| Overall Number Of Images | 1 dataset per animal per imaging technique per time point |
| Field Of View | Max FOV for PET and CT, 14x15 mm for MRI |
| Dimension Extents | PET: 192x192x384; MRI: 256x96x102 |
| Size Description | PET: 76.8 x 76.8 x 154 mm; MRI: 18,7 x 7 x 15 mm |
| Pixel Voxel Size Description | PET: 400 µm isometric; MRI: 73 x 73 x 600 µm |
| Image Processing Methods | CT-based normalization |
| Image Reconstruction Algorithm | PET: OSEM (30 iterations); CT: ISRA (iterative). |
| Quality Control | no |
| Image Registration Algorithm | no |
| Ai Enhanced Algorithm | no |
| Qc Info | n/a |
| Corrections | PET: acquisition decay, randoms; CT: none |
| Spatial And Temporal Alignment | PET and CT images were automatically co-registered by the software; MR images were co-registered using CT |
| Fiducials Used | Brain volume |
| Coregistered Images | Yes |
| Transformation Matrix Other Info | SPM coregistration |
Analyzed Data
| Analysis Result Type | regional increase of radiotracer's uptake |
|---|---|
| Data Used For Analysis | VOIs analysis, p value |
| Analysis Method And Details | SPM, PMOD |
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C103512,NCIT:C16809 |
|---|---|
| Ncit Imaging Submodality | NCIT:C180729 |
| Doid | DOID:14330 |
| Ncit Anatomy | NCIT:C12439 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C14424 |
| Chebi Pharmaco | CHEBI:17963 |
| Chebi Anesthesia | CHEBI:6015 |