Metadata
Dataset ID:
9
Study Design
| Study Design Background | Evaluation of new MRI-CEST contrast agent based on glucose |
|---|---|
| Study Description | GLINT glucose-3OMG comparison |
| Study Type | preclinical |
| Study Subtype | in vivo |
Publication
| Paper Linked | yes |
|---|---|
| Paper Title | In vitro and in vivo comparison of MRI chemical exchange saturation transfer (CEST) properties between native glucose and 3-O-Methyl-D-glucose in a murine tumor model |
| Paper Authors | Anemone A, Capozza M, Arena F, Zullino S, Bardini P, Terreno E, Longo DL, Aime S. |
| Affiliation | Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy; Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy. |
| Paper Journal | NMR Biomed |
| Paper Year | 2021 |
| Paper Doi | https://doi.org/10.1002/nbm.4602 |
| Open Access | yes |
| Updated Year | 2022 |
Study Component
| Multi Modality Images | no |
|---|---|
| Imaging Modality | MRI |
| Imaging Sub Modality | CEST |
| Radiation | no |
| Imaging Coverage | body |
| Imaging Target | glucose transporter (GLUT) |
Dataset Information
| Institution | IBB-CNR |
|---|---|
| Pi | Dario Longo |
| Country Of Institution | Italy |
| Euro Bio Imaging Node | MMMI |
| Country Of Imaging Facility | Italy |
| Contact Person | dario.longo@unito.it |
| Link To Dataset1 | https://eubi-xnat.hpc4ai.unito.it/app/template/XDATScreen_report_xnat_projectData.vm/search_element/xnat:projectData/search_field/xnat:projectData.ID/search_value/glint_glucose-3OMG |
In Vivo Experimental Parameters
| Number Of Groups | 4 |
|---|---|
| Types Of Groups | glucose injected low dose, glucose injected high dose, 3OMG injected low dose, 3OMG injected high dose |
| Overall Sample Size | 20 |
| Disease Model | cancer |
| Organ Or Tissue | melanoma |
| Sample Size For Each Group | 5, 5, 5, 5 |
| Power Calculation | none |
| Randomization | no |
| Blinding | no |
| Outcome Measures | CEST contrast, tumor volume |
| Statistical Methods | unpaired Student's t-test |
| Species | Mice |
| Strain | C57/BL6 |
| Immune Status | Immunocompetent |
| Sex | male |
| Age | 8 weeks |
| Age At Start Experiment | 8 weeks |
| Weight | 25 g |
| Source Of Animals | Charles River Laboratories, Calco, Ital |
Experimental Procedures
| Pharmacological Procedures Intervention And Control | no |
|---|---|
| Blood Sampling | no |
| Surgical Procedures Including Sham Surgery | no |
| Pathogen Infection Intervention And Control | no |
| Analgesic Plan To Relieve Pain Suffering And Distress | no |
| Anesthesia Type | gas |
| Duration | 45 |
| Anesthesia Drugs | Isofluran |
| Anesthesia Dose | 1-2% |
| Monitoring Regime | respiratoy rate |
| Euthanasia | yes |
| Method | cervical dislocation |
| Histology | no |
| Frequency Of Imaging | single scan |
| Overall Scan Length | 45 |
| Contrast Agent Or Radio Isotope Or Challenge With Gas Molecule | yes |
| Contrast Agent Commercial Drug | glucose, 3OMG |
| Contrast Agent Chemical Drug | glucose, 3OMG |
| Contrast Agent Dose | 1.5 g/kg, 3.0 g/kg |
| Injection Volume | 0,2 |
| Injection Time | 20 seconds |
| Vehicle | saline |
| Route Of Administration | intravenous |
| Cell Line | B16-F10 |
| Provenance | collaborator |
| Cell Injection Route | subcutaneous |
| Number Of Cells | 500000 |
| Housing Room | not SPF |
| Dietary Intervention | no |
| Food Intake Measured | no |
Image Acquisition
| Instrument Vendor | Bruker |
|---|---|
| Instrument Type | Pharmascan 70/16, Biospec |
| Instrument Specifics | 7T, 3T |
| Correction | no |
| Raw Data | yes |
| Qa Qc | no |
Image Correlation
| Image Type | Primary image/processed images |
|---|---|
| Format Compression | raw; DICOM |
| Dimensions | 2D + time |
| Field Of View | 30x30x2 mm |
| Dimension Extents | 64x64x1 |
| Size Description | 30x30x2 mm |
| Pixel Voxel Size Description | 0.468x0.468x2 mm |
| Image Processing Methods | default reconstruction |
| Quality Control | no |
| Image Smoothing Or Filtering Algorithm | n/a |
| Image Registration Algorithm | no |
| Ai Enhanced Algorithm | yes |
| Qc Info | R2 calculation on raw data, CEST contrast |
Analyzed Data
| Analysis Result Type | Numerical |
|---|---|
| Data Used For Analysis | CEST contrast |
| Analysis Method And Details | Manual delineation of tumor regions |
| Status | complete |
Ontology Terms
| Ncit Imaging | NCIT:C16809 |
|---|---|
| Ncit Imaging Submodality | NCIT:C116451 |
| Doid | DOID:1909 |
| Ncit Species | NCIT:C14238 |
| Ncit Strain | NCIT:C14424 |
| Chebi Anesthesia | CHEBI:6015 |
| Chebi Contrast Agent Commercial Name | CHEBI:73918 |
| Chebi Contrast Agent Chemical Name | CHEBI:73918 |
| Clo | CLO_0001794 |